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| PolysacDB ID | 1093 |
| Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 4 (reference strain M62) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 6D11 |
| Antibody type and class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab showed a high degree of reactivity with serotypes 4 and 7 and also detected some isolates belonging to serotypes 1, 2, 3, 6, and 8, as well as some nontypeable isolates |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 28-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1094 |
| Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 4 (reference strain M62) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 6B4 |
| Antibody type and class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab reacted with only 24.2 and 13.8% of homologous isolates, respectively, but detected 63% of the serotype 7 isolates. A minor reactivity was observed with other serotypes |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 28-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1095 |
| Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 4 (reference strain M62) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 5E4 |
| Antibody type and class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab reacted with only 24.2 and 13.8% of homologous isolates, respectively, but detected 63% of the total 12 serotype 7 isolates. A minor reactivity was observed with other serotypes |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 28-01-2010 |
| References | PMC170200 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |