PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 7
Entry No. - 1 [TOP]

PolysacDB ID	1142
Carbohydrate Name	Capsular Polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature used to produce antibodies	Whole cells of B. fragilis NCTC 9343
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab BF4
Antibody type and class	IgM
Assay System	ELISA, Enzyme Immunoassay and immunoblotting
Cross-reactivity	MAb Bf4 did not cross react with the sixteen Bacteroides species and strains tested
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-02-2010
References	3309142

Entry No. - 2 [TOP]

PolysacDB ID	1143
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed functions	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature used to produce antibodies	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab QUBf5
Antibody type and class	IgG2b
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross-reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE3
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	15-02-2010
References	1719202

Entry No. - 3 [TOP]

PolysacDB ID	1144
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed functions	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature used to produce antibodies	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab QUBf6
Antibody type and class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross-reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE4
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	15-02-2010
References	1719202

Entry No. - 4 [TOP]

PolysacDB ID	1145
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed functions	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature used to produce antibodies	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab QUBf7
Antibody type and class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross-reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE5
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-02-2010
References	1719202

Entry No. - 5 [TOP]

PolysacDB ID	1146
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed functions	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature used to produce antibodies	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab QUBf8
Antibody type and class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross-reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE6
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-02-2010
References	1719202

Entry No. - 6 [TOP]

PolysacDB ID	1152
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab QUBF 12
Antibody type and class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross-reactivity	This Mab cross-reacted with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. All reacted with B. fragilis strain BE4
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may help in the elucidation of variability of surface polysaccharide structures
Curator ID	AA + AS
Date of Curation	18-02-2010
References	PMC96751

Entry No. - 7 [TOP]

PolysacDB ID	1155
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The polysaccharide part of the LPS mainly consists of two fractions : PSI : β-D-Galp(1-->6)β-D-Galp(1-->6)β-D-Galp(1-->6)Residue1-α-D-Glcp [branched to Residue no. 2](1-->2)α-L-Rhap and PSII : β-D-Galp(1-->4)α-D-Glcp [Branched to β-D-Galp(1-->6)](1-->2)α-L-Rhap
BCSDB Structure	N/A
Proposed functions	LPS polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature used to produce antibodies	Whole cells of B. fragilis NCTC 9343
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Polysera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	This antisera cross-reacted with 13 strains of B. fragilis
Proposed epitopes	Galactose chains was proposed to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This antisera can be used in serotyping schemes for this particular bacteria
Curator ID	AA + AS
Date of Curation	19-02-2010
References	PMC262078

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