PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1163
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBordetella pertusis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureB. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B
BCSDB StructureN/A
Proposed functionsThe LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties
Antigenic Nature used to produce antibodiesWhole cells of Bordetella Pertusis
Carrier NameNil
Conjugation MethodNil
AntibodiesMab HBp2
Antibody type and classIgM
Assay SystemDot enzyme Immunoassay, Immunoblotting
Cross-reactivityThis antibody reacted with all B. pertusis strains. also cross-reacted with a clinical isolate of B. bronchiseptica. No cross-reaction was observed against B. parapertussis and other gram-negative species
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityMab HBp2 showed high lytic capability against B. pertussis
Curator IDAA + AS
Date of Curation23-02-2010
References1721552


Entry No. - 2   [TOP]
PolysacDB ID1171
Carbohydrate NameLipoologosaccharide band A of the LPS   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBordetella pertusis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureB. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B
BCSDB StructureN/A
Proposed functionsThe LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties
Antigenic Nature used to produce antibodiesN/A
Carrier NameN/A
Conjugation MethodN/A
AntibodiesMab G10F8C3
Antibody type and classIgG3
Assay SystemImmunoblotting, Protein A sepharose affinity chromatography, bactericidal assays
Cross-reactivityThis antibody was specific to B. pertusis strains
Proposed epitopesThis Mab reacts with the core oligosaccharide moeity
IEDB EpitopeN/A
Proposed UtilityThis Mab exhibits complement dependent bactericidal activity against strains of B. pertussis that express the LOS A phenotype. In addition it was effective in reducing colonization by B. pertussis in both the lungs and tracheas of mice after aerosol infection
Curator IDAA + AS
Date of Curation25-02-2010
ReferencesPMC258314


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036