PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 5

Entry No. - 1   [TOP]
PolysacDB ID1232
Carbohydrate NameCore oligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus S19   (NCBI Taxonomy)   (Drugpedia)
Basic StructureCore oligosaccharide is composed of mannose, glucose, 2-amino-2,6-dideoxyglucose (quinovosamine), 2-amino-2-deoxyglucose (GlcN),3-deoxy-D-manno-2-octulosonic acid (KDO), and unidentified sugars. The core is linked to the lipid A, which in contrast to most LPS studied, contains both GlcN and 2,3- diamino-2,3-dideoxyglucose (GlcN3N) as backbone sugars and amide- and ester-linked long-chain saturated (C16:0 to C18:0) and hydroxylated (3-OH-C12:0, to 29-OH-C30:0) fatty acids
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAcetone-killed B. abortus S19 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Baro-1
Antibody type and classIgG3
Assay SystemEnzyme Immunoassay,immunoblotting
Cross-reactivityBaro1 Mab showed cross-reactivity against B. melitiensis, B. canis and B. ovis LPS. Did not cross-react wirth S. coli and S. minnesota
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator IDAA + AS
Date of Curation27-03-2010
ReferencesPMC368229


Entry No. - 2   [TOP]
PolysacDB ID1233
Carbohydrate NameCore oligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus S19   (NCBI Taxonomy)   (Drugpedia)
Basic StructureCore oligosaccharide is composed of mannose, glucose, 2-amino-2,6-dideoxyglucose (quinovosamine), 2-amino-2-deoxyglucose (GlcN),3-deoxy-D-manno-2-octulosonic acid (KDO), and unidentified sugars. The core is linked to the lipid A, which in contrast to most LPS studied, contains both GlcN and 2,3- diamino-2,3-dideoxyglucose (GlcN3N) as backbone sugars and amide- and ester-linked long-chain saturated (C16:0 to C18:0) and hydroxylated (3-OH-C12:0, to 29-OH-C30:0) fatty acids
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAcetone-killed B. abortus S19 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Baro-2
Antibody type and classIgM
Assay SystemEnzyme Immunoassay,immunoblotting
Cross-reactivityThe Baro-2 MAb showed reactivity against LPS from E. coli Re, S. minnesota Re, S. typhimurium Rd2, and S. typhimurium Rdl
Proposed epitopes3-deoxy-D-manno-2-octulosonic acid (KDO)seems to be an important part of the epitope
IEDB EpitopeN/A
Proposed UtilityThis Mab may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator IDAA + AS
Date of Curation27-03-2010
ReferencesPMC368229


Entry No. - 3   [TOP]
PolysacDB ID1234
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus S19   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharide consists of an unbranched linear homopolymer of 10 to 100 α-1,2-linked 4-formamido-4,6-dideoxy-D-mannose (N formylperosamine) residues. The O-polysaccharide is covalently linked to a core oligosaccharide
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAcetone-killed B. abortus S19 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Baps-A
Antibody type and classIgG3
Assay SystemEnzyme Immunoassay,immunoblotting
Cross-reactivityThis antibody cross-reacted with Y. enterocolitica 0:9 LPS. Did not cross-react with core oligosaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator IDAA + AS
Date of Curation28-03-2010
ReferencesPMC368229


Entry No. - 4   [TOP]
PolysacDB ID1235
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus S19   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharide consists of an unbranched linear homopolymer of 10 to 100 α-1,2-linked 4-formamido-4,6-dideoxy-D-mannose (N formylperosamine) residues. The O-polysaccharide is covalently linked to a core oligosaccharide
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAcetone-killed B. abortus S19 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Baps C/Y
Antibody type and classIgG3
Assay SystemEnzyme Immunoassay,immunoblotting
Cross-reactivityThis antibody cross-reacted with Y. enterocolitica 0:9 LPS. Did not cross-react with core oligosaccharide
Proposed epitopesThis Mab recognized the commom [C] epitope of Brucella abortus LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator IDAA + AS
Date of Curation28-03-2010
ReferencesPMC368229


Entry No. - 5   [TOP]
PolysacDB ID1236
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus S19   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS mainly consists of O-polysaccharide and core oligosaccharide
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodies B. abortus S19 infection
Carrier NameNil
Conjugation MethodNil
AntibodiesBovine antisera
Antibody type and classN/A
Assay SystemEnzyme Immunoassay,immunoblotting
Cross-reactivityThis antibody cross-reacted with B. melitensis 16M polysaccharide native hapten (NH)
Proposed epitopesThe predominant epitopes were proposed to be a and C epitopes
IEDB EpitopeN/A
Proposed UtilityThis antisera may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator IDAA + AS
Date of Curation28-03-2010
ReferencesPMC368229


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