PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1239
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus W99   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharide consists of an unbranched linear homopolymer of 10 to 100 α-1,2-linked 4-formamido-4,6-dideoxy-D-mannose (N formylperosamine) residues. The O-polysaccharide is covalently linked to a core oligosaccharide
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole B. abortus W99 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 4F9
Antibody type and classIgG3
Assay SystemELISA
Cross-reactivityThis antibody did not cross-react with S-LPSs of S. urbana, Vibrio cholerae and E. coli O:157, but cross-reacted with S-LPS of Y. enterocolitica O:9 along with Brucella A and M dominant strains. Reactivity was more towards A dominant strains
Proposed epitopesThis Mab was directed against the C/Y epitope and did not consist of α1,2-linked D-Rha4NFo residues. This Mab did not bind purified O-polysaccharide
IEDB EpitopeN/A
Proposed UtilityThis Mab helped in the epitope strucure elucidation
Curator IDAA + AS
Date of Curation03-04-2010
ReferencesPMC175246


Entry No. - 2   [TOP]
PolysacDB ID1242
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeBrucella abortus W99   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharide consists of an unbranched linear homopolymer of 10 to 100 α-1,2-linked 4-formamido-4,6-dideoxy-D-mannose (N formylperosamine) residues. The O-polysaccharide is covalently linked to a core oligosaccharide
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole B. abortus W99 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 2C8C4
Antibody type and classIgG3
Assay SystemELISA
Cross-reactivityThis Mab was specific to A strains of Brucella and did not cross-react with Brucella M strain, Y enterocolitica O:9, S-LPSs of S.urbana, Vibrio cholerae and E. coli O:157
Proposed epitopesThe epitope was proposed to be a pentasaccharide or larger oligosaccharide composed of α1,2-linked D-Rha4NFo and also contained α1,3 linkages
IEDB EpitopeN/A
Proposed UtilityThis Mab helped in the epitope strucure elucidation
Curator IDAA + AS
Date of Curation04-04-2010
ReferencesPMC175246


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