PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 36
Entry No. - 1 [TOP]

PolysacDB ID	1002
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates.
Carrier Name	Tetanus toxoid
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 2E9
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A, B and D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections
Curator ID	AA + AS
Date of Curation	01-01-2010
References	PMC173409

Entry No. - 2 [TOP]

PolysacDB ID	1006
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 3B6
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotypes A, B, C and D
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections
Curator ID	AA + AS
Date of Curation	02-01-2010
References	PMC173409

Entry No. - 3 [TOP]

PolysacDB ID	1016
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 21D2
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A, D and GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

Entry No. - 4 [TOP]

PolysacDB ID	1017
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 14A12
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

Entry No. - 5 [TOP]

PolysacDB ID	1018
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 11E2
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

Entry No. - 6 [TOP]

PolysacDB ID	1019
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 7B13
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

Entry No. - 7 [TOP]

PolysacDB ID	1020
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 12G5
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

Entry No. - 8 [TOP]

PolysacDB ID	1021
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 20C5
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

Entry No. - 9 [TOP]

PolysacDB ID	1022
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 20B5
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

Entry No. - 10 [TOP]

PolysacDB ID	1023
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 4H3
Antibody type and class	IgG3
Assay System	ELISA
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC2118886

Entry No. - 11 [TOP]

PolysacDB ID	1024
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Cryptococci cells
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 15C6
Antibody type and class	IgA
Assay System	Immunofluorescence
Cross-reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC2118886

Entry No. - 12 [TOP]

PolysacDB ID	1025
Carbohydrate Name	Capsular polysaccharide A (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	N/A
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Sheep erythrocytes
Conjugation Method	The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
Antibodies	Mab 439
Antibody type and class	IgG1
Assay System	Ouchterlony double immunodiffusion, Phagocytosis assays
Cross-reactivity	This antibody cross-reacted with all the 4 serotypes- A, B, C and D
Proposed epitopes	The reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC260621

Entry No. - 13 [TOP]

PolysacDB ID	1026
Carbohydrate Name	Capsular polysaccharide A (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	N/A
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Sheep erythrocytes
Conjugation Method	The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
Antibodies	Mab 1255
Antibody type and class	IgG1
Assay System	Ouchterlony double immunodiffusion, Phagocytosis assays
Cross-reactivity	This antibody cross-reacted with 3 serotypes- A, B and D
Proposed epitopes	The reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	08-01-2010
References	PMC260621

Entry No. - 14 [TOP]

PolysacDB ID	1027
Carbohydrate Name	Capsular polysaccharide D (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	N/A
Proposed functions	The capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Sheep erythrocytes
Conjugation Method	The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
Antibodies	Mab 302
Antibody type and class	IgG1
Assay System	Ouchterlony double immunodiffusion, Phagocytosis assays
Cross-reactivity	This antibody cross-reacted with 2 serotypes- A and D
Proposed epitopes	The reactivity of this antibody required acetylation of the parent polysaccharide. Hence proposed epitopes may be O-acetyl groups
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	08-01-2010
References	PMC260621

Entry No. - 15 [TOP]

PolysacDB ID	1028
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	130362
Proposed functions	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugation Method	GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg]
Antibodies	Mab BD1
Antibody type and class	IgG1
Assay System	Indirect immunofluorescence
Cross-reactivity	This antibody cross-reacted with 2 serotypes- A and D. BD-1 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus
Proposed epitopes	Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	08-01-2010
References	PMC259921

Entry No. - 16 [TOP]

PolysacDB ID	1029
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	130362
Proposed functions	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugation Method	GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg]
Antibodies	Mab BA4
Antibody type and class	IgM
Assay System	Indirect immunofluorescence
Cross-reactivity	This antibody cross-reacted with 2 serotypes- A and B. BA4 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	08-01-2010
References	PMC259921

Entry No. - 17 [TOP]

PolysacDB ID	1031
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	130362
Proposed functions	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 18B7
Antibody type and class	IgG1
Assay System	Invivo protection assay
Cross-reactivity	This antibody cross-reacted with all 4 serotypes- A, B, C and D.
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody opsonized Serotypes A and D and was shown to activate the complement pathway. This antibody is in pre-clinical development for treatment of Cryptococcus neoformans infections
Curator ID	AA + AS
Date of Curation	09-01-2010
References	PMC105619

Entry No. - 18 [TOP]

PolysacDB ID	2617
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 4H9
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	21-07-2011
References	10456888

Entry No. - 19 [TOP]

PolysacDB ID	2618
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 6C3.4
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	22-07-2011
References	10456888

Entry No. - 20 [TOP]

PolysacDB ID	2619
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 6A5
Antibody type and class	IgG3
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	22-07-2011
References	10456888

Entry No. - 21 [TOP]

PolysacDB ID	2620
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 9A12
Antibody type and class	IgG3
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	22-07-2011
References	10456888

Entry No. - 22 [TOP]

PolysacDB ID	2621
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab A2F12
Antibody type and class	IgG3
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	23-07-2011
References	10456888

Entry No. - 23 [TOP]

PolysacDB ID	2622
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 6C3.3
Antibody type and class	IgG1
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	23-07-2011
References	10456888

Entry No. - 24 [TOP]

PolysacDB ID	2623
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab IG5
Antibody type and class	IgG2a
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	24-07-2011
References	10456888

Entry No. - 25 [TOP]

PolysacDB ID	2624
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 7f8
Antibody type and class	IgG2a
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	24-07-2011
References	10456888

Entry No. - 26 [TOP]

PolysacDB ID	2625
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 12g6
Antibody type and class	IgG2a
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	24-07-2011
References	10456888

Entry No. - 27 [TOP]

PolysacDB ID	2626
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 12A1.4
Antibody type and class	IgG2b
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	24-07-2011
References	10456888

Entry No. - 28 [TOP]

PolysacDB ID	2627
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 15E6
Antibody type and class	IgG2b
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	24-07-2011
References	10456888

Entry No. - 29 [TOP]

PolysacDB ID	2628
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 14F8
Antibody type and class	IgG2b
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	25-07-2011
References	10456888

Entry No. - 30 [TOP]

PolysacDB ID	2629
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 2G3
Antibody type and class	IgA
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	25-07-2011
References	10456888

Entry No. - 31 [TOP]

PolysacDB ID	2630
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 13C8
Antibody type and class	IgA
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	25-07-2011
References	10456888

Entry No. - 32 [TOP]

PolysacDB ID	2631
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 16
Antibody type and class	IgG1
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	26-07-2011
References	10456888

Entry No. - 33 [TOP]

PolysacDB ID	2632
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 23
Antibody type and class	IgG1
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	26-07-2011
References	10456888

Entry No. - 34 [TOP]

PolysacDB ID	2633
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 25
Antibody type and class	IgG1
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	26-07-2011
References	10456888

Entry No. - 35 [TOP]

PolysacDB ID	2634
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 29
Antibody type and class	IgG1
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	27-07-2011
References	10456888

Entry No. - 36 [TOP]

PolysacDB ID	2635
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	130362
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Mab 12A1
Antibody type and class	IgM
Assay System	ELISA
Cross-reactivity	This antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice
Curator ID	AA + AS
Date of Curation	27-07-2011
References	10456888

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036