PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID2601
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCryptococcus neoformans Serotype B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure130477
Proposed functionsThe capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameSheep erythrocytes
Conjugation MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 339
Antibody type and classN/A
Assay SystemELISA and Immunodiffusion
Cross-reactivityThis antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes
Curator IDAA + AS
Date of Curation15-07-2011
References2476401


Entry No. - 2   [TOP]
PolysacDB ID2637
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCryptococcus neoformans Serotype B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure9354
Proposed functionsAntiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameSheep erythrocytes
Conjugation MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab F10F5
Antibody type and classIgG1
Assay SystemELISA
Cross-reactivityThis antibody cross-reacted with Serotypes A and B
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan
Curator IDAA + AS
Date of Curation28-07-2011
References12965925


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