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| PolysacDB ID | 2602 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype D (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 136943 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugation Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 386 |
| Antibody type and class | N/A |
| Assay System | ELISA and Immunodiffusion |
| Cross-reactivity | This antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA |
| Proposed epitopes | Since Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes |
| Curator ID | AA + AS |
| Date of Curation | 15-07-2011 |
| References | 2476401 |
| PolysacDB ID | 2605 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype D (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 136943 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Keyhole limpet hemocyanin |
| Conjugation Method | Forty milligrams of crude polysaccharide was dissolved in 4 ml of physiological saline solution (PSS). One milliliter of 1% cyanuric chloride in dimethylformamide was added to the polysaccharide solution and stirred for 1 h at 48°C. KLH in PSS (100 mg/2ml) was added and stirred for 1 h at 258°C, and the solution was then incubated at 48C for 18 h. The solution was dialyzed against water for 30 h and centrifuged. The protein concentration in the supernatant was adjusted to 2 mg/ml, 1/9 volume of 10% aluminum potassium sulfate solution was added, and the pH was adjusted to 6.5 with 10% sodium carbonate. The precipitated antigen was removed by centrifugation and resuspended in PSS |
| Antibodies | Mab CRND8 |
| Antibody type and class | IgM |
| Assay System | ELISA and CSA[Cell Slide Agglutination] |
| Cross-reactivity | This antibody cross-reacted with Serotype A and D serotypes |
| Proposed epitopes | O-acetylated glucurunoxylomannan |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | PMC170253 |
| PolysacDB ID | 2642 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype D (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | This polysaccharide is a high m.w. polymer with a linear α-(1-->3)-linked mannose backbone that is substituted with non-reducing D-xyIosyal and D-glucosyluronic acid groups. O-Acetylation varies with the serotype. Type D is the most heavily O-acetylated |
| BCSDB Structure | 136943 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Methylated bovine serum albumin |
| Conjugation Method | A saline solution containing 500 pg of cryptococcal polysaccharide was mixed with 500 pg aqueous methylated bovine serum albumin. The volume was brought to 2 ml with sterile distilled water |
| Antibodies | Polysera |
| Antibody type and class | IgG |
| Assay System | Antiserum to untreated cryptococcal polysaccharide was enriched for O-acetyl and carboxyl specific antibody respectively. The antisera was then used in ouchterlony double diffusion. |
| Cross-reactivity | N/A |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | O-acetyl and carboxyl groups are strong and distinct physicochemical determinants on cryptococcal polysaccharide. The enriched antiserum displayed a level of opsonic activity that was similar to non-enriched antiserum. This showed that neither O-acetyl nor carboxyl groups were responsible for inhibition of phagocytosis by cryptococcal polysaccharide |
| Curator ID | AA + AS |
| Date of Curation | 30-07-2011 |
| References | 15271943 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |