PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 8
Entry No. - 1 [TOP]

PolysacDB ID	1489
Carbohydrate Name	Heptasaccharide motif (M2) of Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The heptasaccharide consists of two M2 triad repeats and mannose residue. The M2 repeat was selected because it is commonly found in GXM of serotype A strains
BCSDB Structure	N/A
Proposed functions	Glucurunoxylomannan is an antiphagocytic determinant ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Human serum albumin
Conjugation Method	N/A
Antibodies	Mab 1H1
Antibody type and class	IgA
Assay System	Immunofluorescence and phagocytosis assays
Cross-reactivity	This antibody cross-reacted with Serotype D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	02-08-2010
References	19464529

Entry No. - 2 [TOP]

PolysacDB ID	1490
Carbohydrate Name	Heptasaccharide motif (M2) of Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The heptasaccharide consists of two M2 triad repeats and mannose residue. The M2 repeat was selected because it is commonly found in GXM of serotype A strains
BCSDB Structure	N/A
Proposed functions	Glucurunoxylomannan is an antiphagocytic determinant ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Human serum albumin
Conjugation Method	N/A
Antibodies	Mab 5H8
Antibody type and class	IgM
Assay System	Immunofluorescence and phagocytosis assays
Cross-reactivity	This antibody cross-reacted with Serotype D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	02-08-2010
References	19464529

Entry No. - 3 [TOP]

PolysacDB ID	1491
Carbohydrate Name	Heptasaccharide motif (M2) of Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The heptasaccharide consists of two M2 triad repeats and mannose residue. The M2 repeat was selected because it is commonly found in GXM of serotype A strain
BCSDB Structure	N/A
Proposed functions	Glucurunoxylomannan is an antiphagocytic determinant ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Human serum albumin
Conjugation Method	N/A
Antibodies	Mab 3F10
Antibody type and class	IgM
Assay System	Immunofluorescence and phagocytosis assays
Cross-reactivity	This antibody cross-reacted with Serotype D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	02-08-2010
References	19464529

Entry No. - 4 [TOP]

PolysacDB ID	1492
Carbohydrate Name	Heptasaccharide motif (M2) of Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The heptasaccharide consists of two M2 triad repeats and mannose residue. The M2 repeat was selected because it is commonly found in GXM of serotype A strain
BCSDB Structure	N/A
Proposed functions	Glucurunoxylomannan is an antiphagocytic determinant ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Human serum albumin
Conjugation Method	N/A
Antibodies	Mab 1D6
Antibody type and class	IgM
Assay System	Immunofluorescence and phagocytosis assays
Cross-reactivity	This antibody cross-reacted with Serotype D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-08-2010
References	19464529

Entry No. - 5 [TOP]

PolysacDB ID	1493
Carbohydrate Name	Heptasaccharide motif (M2) of Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The heptasaccharide consists of two M2 triad repeats and mannose residue. The M2 repeat was selected because it is commonly found in GXM of serotype A strain
BCSDB Structure	N/A
Proposed functions	Glucurunoxylomannan is an antiphagocytic determinant ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Human serum albumin
Conjugation Method	N/A
Antibodies	Mab 1E3
Antibody type and class	IgM
Assay System	Immunofluorescence and phagocytosis assays
Cross-reactivity	This antibody cross-reacted with Serotype D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-08-2010
References	19464529

Entry No. - 6 [TOP]

PolysacDB ID	2639
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	N/A
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Diphtheria toxoid
Conjugation Method	5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
Antibodies	Mab G14
Antibody type and class	IgM
Assay System	ELISA and immunofluorescence
Cross-reactivity	This antibody cross-reacted with Serotypes A and D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb mediated complement deposition on solid phase GXM by ELISA
Curator ID	AA + AS
Date of Curation	28-07-2011
References	15271943

Entry No. - 7 [TOP]

PolysacDB ID	2640
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	N/A
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Diphtheria toxoid
Conjugation Method	5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
Antibodies	Mab G15
Antibody type and class	IgM
Assay System	ELISA and immunofluorescence
Cross-reactivity	This antibody cross-reacted with Serotypes A and D in whole cell ELISA and immunofluorescence. Did not cross-react with Serotype A glucurunoxylomannan alone
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb mediated complement deposition on solid phase GXM by ELISA. Administration of Mab G15 also prolonged the survival of C. neoformans
Curator ID	AA + AS
Date of Curation	29-07-2011
References	15271943

Entry No. - 8 [TOP]

PolysacDB ID	2641
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia)
Basic Structure	A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure	N/A
Proposed functions	Antiphagocytic ; an important virulence factor
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Diphtheria toxoid
Conjugation Method	5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
Antibodies	Mab G19
Antibody type and class	IgM
Assay System	ELISA and immunofluorescence
Cross-reactivity	This antibody cross-reacted with Serotypes A and D
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb mediated complement deposition on solid phase GXM by ELISA
Curator ID	AA + AS
Date of Curation	29-07-2011
References	15271943

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