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| PolysacDB ID | 1001 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 116826 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates. |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA), tetanus toxoid |
| Conjugation Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Polysera |
| Antibody type and class | IgM |
| Assay System | Double immunodiffusion, ELISA |
| Cross-reactivity | Polsera cross-reacted with capsular polysaccharides of C neoformans serotype A and D |
| Proposed epitopes | O-acetyl groups, glucuronyl residues |
| IEDB Epitope | 115576 |
| Proposed Utility | The conjugate vaccines prepared through hydroxyl activation of glucurunoxylomannan are sufficiently immunogenic and appear to be suitable for clinical evaluation |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | 1716613 |
| PolysacDB ID | 1013 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM backbone consists of a linear α(1-->3)-linked mannan substituted at 2-O positions by single residues of either xylose or glucuronic acid |
| BCSDB Structure | 116826 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Pure Capsular polysaccharide |
| Carrier Name | N/A |
| Conjugation Method | N/A |
| Antibodies | Mab E1 |
| Antibody type and class | IgG1 |
| Assay System | Agglutination with C. neoformans serotype A cells, Radial immunodiffusion, Indirect Immunofluorescence,, ELISA, Competitive binding assays |
| Cross-reactivity | This antibody was highly specific to serotype A, low levels of cross reactivity to serotypes B and D. Among the other yeasts tested, a cross-reaction was only detected with Trichosporon beigelii. |
| Proposed epitopes | N\A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could be useful for fundamental studies on the glucuronoxylomannan structure, as well as for clinical applications such as serotyping and possibly the serological diagnosis of cryptococcosis |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC260404 |
| PolysacDB ID | 2603 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 130477 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugation Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 471 |
| Antibody type and class | N/A |
| Assay System | ELISA and Immunodiffusion |
| Cross-reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes. This monoclonal antibody promoted opsonization of Cryptococcus cells |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 2476401 |
| PolysacDB ID | 2606 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 2D10 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed epitopes | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2607 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 3E5 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed epitopes | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2609 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 17E12 |
| Antibody type and class | IgG1 |
| Assay System | ELISA |
| Cross-reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed epitopes | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2610 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 18G9 |
| Antibody type and class | IgA |
| Assay System | ELISA |
| Cross-reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed epitopes | This antibody did not bind O-deacetylated glucurunoxylomannan indicating that acetylated residues on glucurunoxylomannan surface are an essential part of the epitope |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 17-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2611 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 2H1 |
| Antibody type and class | IgG1 |
| Assay System | ELISA |
| Cross-reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed epitopes | This antibody did not bind O-deacetylated glucurunoxylomannan indicating that acetylated residues on glucurunoxylomannan surface are an essential part of the epitope |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 17-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2612 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed functions | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugation Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 1326 |
| Antibody type and class | IgG1 |
| Assay System | ELISA |
| Cross-reactivity | This antibody was cross-reactive to serotypes A and D |
| Proposed epitopes | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | This monoclonal antibody promoted opsonization of Cryptococcus cells |
| Curator ID | AA + AS |
| Date of Curation | 18-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2636 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 116826 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | Conjugated using CDAP chemistry |
| Antibodies | Mab 13F1 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed epitopes | This Mab did not bind de-O-acetylated GXM suggesting that acetylated residues form an important part of the epitope |
| IEDB Epitope | 115576 |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 28-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2638 |
| Carbohydrate Name | De-O-acetylated Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 9353 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugation Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab F12D2 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | This antibody cross-reacted with Serotypes A, B, C and D |
| Proposed epitopes | Studies on this antibody showed that there are 2 types of epitopes on GXM-O-acetyl dependent and O-acetyl independent. MAb F12D2 also fails to react with xylose deficient GXM shopwing that xylose residues form an important part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan. This antibody showed that the induction of a MAb that is reactive with all serotypes does not inherently require the presence of an O-acetyl group on the immunizing GXM |
| Curator ID | AA + AS |
| Date of Curation | 28-07-2011 |
| References | 12965925 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |