PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 17
Entry No. - 1 [TOP]

PolysacDB ID	1376
Carbohydrate Name	Capsular polysaccharide 500 antigen (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	CPS-500 is a chloroform-extractable glycolipid
BCSDB Structure	N/A
Proposed functions	CPS-500 is a candidate target antigen for active or passive immunization against cryptosporidiosis
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 18.44
Antibody type and class	IgG3
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The neutralization-sensitive epitope recognized by MAb 18.44 is dependent on terminal β-D-mannopyranosyl residues
IEDB Epitope	N/A
Proposed Utility	MAb 18.44 neutralizes the infectivity of both sporozoites and merozoites and to significantly reduce C. parvum infection in oocyst-challenged mice when coadministered with other neutralizing Mab
Curator ID	AA + AS
Date of Curation	02-07-2010
References	2193057

Entry No. - 2 [TOP]

PolysacDB ID	1377
Carbohydrate Name	Gp40 glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Fixed Cryptosporidium parvum sporozoites
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 4E9
Antibody type and class	IgM
Assay System	Immunofluorescence, immunoblotting
Cross-reactivity	This Mab did not react other protozoa (Giardia lamblia, E. Histolytica, Toxoplasma gondii, Trypanosoma cruzi, and L. major) and was specific for Cryptosporidium
Proposed epitopes	The epitope recognized by 4E9 contains α-N-acetylgalactosamine residues, which are present in a mucin-type O-glycosidic linkage
IEDB Epitope	N/A
Proposed Utility	This MAb neutralized C. parvum infection and inhibited sporozoite attachment to intestinal epithelial cells in vitro
Curator ID	AA + AS
Date of Curation	02-07-2010
References	PMC101770

Entry No. - 3 [TOP]

PolysacDB ID	1378
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Oocyst cell wall preparation
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Cry104
Antibody type and class	IgG1
Assay System	ELISA and immunoblotting
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	Cry104 gave the tightest cluster of oocysts and the best separation from debris in concentrated environmental water samples
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC95949

Entry No. - 4 [TOP]

PolysacDB ID	1379
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Supernatant from sonicated Oocysts
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 7B3
Antibody type and class	N/A
Assay System	Immunoprecipitation and immunofluorescence
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a potential target for passive immunotherapy against cryptosporidiosis
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC258288

Entry No. - 5 [TOP]

PolysacDB ID	1380
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Supernatant from sonicated Oocysts
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 10C6
Antibody type and class	N/A
Assay System	Immunoprecipitation and immunofluorescence
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a potential target for passive immunotherapy against cryptosporidiosis
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC258288

Entry No. - 6 [TOP]

PolysacDB ID	1381
Carbohydrate Name	GP900 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Supernatant from sonicated Oocysts
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab E6
Antibody type and class	N/A
Assay System	Immunoprecipitation and immunofluorescence
Cross-reactivity	N/A
Proposed epitopes	N-linked carbohydrates seem to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab is a potential target for passive immunotherapy against cryptosporidiosis
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC258288

Entry No. - 7 [TOP]

PolysacDB ID	1382
Carbohydrate Name	GP15 glycoprotein (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Oocysts of C. Parvum
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 5C3
Antibody type and class	IgA
Assay System	ELISA, immunoblotting and immunoelectron microscopy
Cross-reactivity	This Mab did not cross-react with Cryptosporidium. Baileyi, Cryptosporidium muris, Cryptosporidium serpentis, Besnoitia darlingi,Caryospora bigenetica, Eimeria nieschulzi, E. papillata and Toxoplasma gondii
Proposed epitopes	N-acetylglucosamine residues seemed to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab showed invivo protection against Chlamydia infection
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC258359

Entry No. - 8 [TOP]

PolysacDB ID	1383
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Postexcystation preparation obtained from oocysts
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TOU
Antibody type and class	IgG
Assay System	Immunoelectron microscopy and immunoblotting
Cross-reactivity	This Mab cross-reacted with 6 different strains of Cryptosporidium parvum
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will help in the elucidation of cellular localization of micronemes and its glycoproteinic nature
Curator ID	AA + AS
Date of Curation	04-07-2010
References	PMC257905

Entry No. - 9 [TOP]

PolysacDB ID	1394
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	C. parvum oocyst extracts
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3D8 2B11
Antibody type and class	IgA
Assay System	ELISA
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	08-07-2010
References	PMC96563

Entry No. - 10 [TOP]

PolysacDB ID	1395
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	C. parvum oocyst extracts
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3F10 1G3
Antibody type and class	IgA
Assay System	ELISA
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	08-07-2010
References	PMC96563

Entry No. - 11 [TOP]

PolysacDB ID	1396
Carbohydrate Name	CSL antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified CSL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3E2
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	09-07-2010
References	PMC97466

Entry No. - 12 [TOP]

PolysacDB ID	1397
Carbohydrate Name	GP25-200 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified GP25-200
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3H2
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	10-07-2010
References	PMC97466

Entry No. - 13 [TOP]

PolysacDB ID	1398
Carbohydrate Name	GP25-200 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified GP25-200 antigen
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 1B3
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	10-07-2010
References	PMC97466

Entry No. - 14 [TOP]

PolysacDB ID	1399
Carbohydrate Name	GP25-200 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified CSL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 1C5
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	10-07-2010
References	PMC97466

Entry No. - 15 [TOP]

PolysacDB ID	1400
Carbohydrate Name	GP25-200 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified CSL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 3D9
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	11-07-2010
References	PMC97466

Entry No. - 16 [TOP]

PolysacDB ID	1401
Carbohydrate Name	GP25-200 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified CSL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 4E11
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	11-07-2010
References	PMC97466

Entry No. - 17 [TOP]

PolysacDB ID	1402
Carbohydrate Name	GP25-200 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Immunoaffinity purified CSL
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 4H9
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, immunofluorescence and invivo protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was able to neutralize cryptosporidium infection
Curator ID	AA + AS
Date of Curation	13-07-2010
References	PMC97466

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