PolysacDB ID | 1449 |
Carbohydrate Name | Capsular polysaccharide [heteroglycan] (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe | Enterococci faecium B210860 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
|
BCSDB Structure | N/A |
Proposed functions | These polysaccharides are major virulence factors |
Antigenic Nature used to produce antibodies | Glycoconjugates |
Carrier Name | Tetanus toxoid |
Conjugation Method | PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4 |
Antibodies | Polysera |
Antibody type and class | N/A |
Assay System | Competitive inhibition ELISA |
Cross-reactivity | This antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001 |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 13-07-2010
|
References | PMC1538600 |