PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1449
Carbohydrate NameCapsular polysaccharide [heteroglycan]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeEnterococci faecium B210860   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
BCSDB StructureN/A
Proposed functionsThese polysaccharides are major virulence factors
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameTetanus toxoid
Conjugation MethodPSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4
AntibodiesPolysera
Antibody type and classN/A
Assay SystemCompetitive inhibition ELISA
Cross-reactivityThis antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation13-07-2010
ReferencesPMC1538600


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036