PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 3
Entry No. - 1 [TOP]

PolysacDB ID	1067
Carbohydrate Name	Enterobacterial common antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Escherichia coli C600 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a glycophospholipid built up by an amino sugar heteropolymer linked to an L-glycerophosphatidyl residue.
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole formalinized E. coli C600 cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 865
Antibody type and class	IgM
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody cross-reacted with purified ECA from S.montevideo and to E. coli Rl and R4 LPS, cross-reacted well with the K5 capsular polysaccharide
Proposed epitopes	Epitope was proposed to contain 4-linked α-N-acetylglucosamine
IEDB Epitope	N/A
Proposed Utility	This Mab may be used for the detection of ECA and K5 core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator ID	AA + AS
Date of Curation	20-01-2010
References	PMC261975

Entry No. - 2 [TOP]

PolysacDB ID	1068
Carbohydrate Name	Enterobacterial common antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Escherichia coli C600 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a glycophospholipid built up by an amino sugar heteropolymer linked to an L-glycerophosphatidyl residue.
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole formalinized E. coli C600 cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 898
Antibody type and class	IgG2a
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody did not cross-react with K5 capsular polysaccharide
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may be used for the detection of ECA core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator ID	AA + AS
Date of Curation	20-01-2010
References	PMC261975

Entry No. - 3 [TOP]

PolysacDB ID	1069
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli C600 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole formalinized E. coli C600 cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 786
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody cross-reacted with LPS core types K-12, R4, and R2 but did not cross-react with Rl LPS and R3 LPS,
Proposed epitopes	The epitope was proposed to consist of trisaccharide sequence Hexp(1->2)-α-D -Glcp(1->3)α-D-Glcp->(Hepp) at the inner region of the outer core, in which the heptose-distal glucose is immunodominant
IEDB Epitope	N/A
Proposed Utility	This Mab may be used for the detection of LPS core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator ID	AA + AS
Date of Curation	21-01-2010
References	PMC261975

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036