PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 7
Entry No. - 1 [TOP]

PolysacDB ID	1073
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 14
Antibody type and class	IgM
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	22-01-2010
References	PMC261046

Entry No. - 2 [TOP]

PolysacDB ID	1074
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 19
Antibody type and class	IgG3
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	This Mab had invitro opsonophagocytic activity and showed complete protection in invivo models
Curator ID	AA + AS
Date of Curation	23-01-2010
References	PMC261046

Entry No. - 3 [TOP]

PolysacDB ID	1075
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 26
Antibody type and class	IgG1
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	This Mab had invitro opsonophagocytic activity and showed complete protection in invivo models
Curator ID	AA + AS
Date of Curation	23-01-2010
References	PMC261046

Entry No. - 4 [TOP]

PolysacDB ID	1076
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 35
Antibody type and class	IgM
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	This Mab showed partial protection in invivo models
Curator ID	AA + AS
Date of Curation	23-01-2010
References	PMC261046

Entry No. - 5 [TOP]

PolysacDB ID	1077
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 39
Antibody type and class	IgM
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	23-01-2010
References	PMC261046

Entry No. - 6 [TOP]

PolysacDB ID	1078
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 109
Antibody type and class	IgM
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	This Mab showed partial protection in invivo models
Curator ID	AA + AS
Date of Curation	24-01-2010
References	PMC261046

Entry No. - 7 [TOP]

PolysacDB ID	1079
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18:K1:H7 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 141
Antibody type and class	IgM
Assay System	ELISA, invitro opsonophagocytosis assay, immunoblotting, invivo protection studies
Cross-reactivity	This Mab cross-reacted with other 018 E. coli strains but were unreactive with heterologous 0 serotypes (e.g., 075 E. coli), E. coli J-5 Rc, Salmonella typhimurium Re rough mutants, or other gram-negative bacteria.
Proposed epitopes	The epitope was proposed to consist of the O-antigen
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	24-01-2010
References	PMC261046

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