PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 6
Entry No. - 1 [TOP]

PolysacDB ID	1080
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide part consists of the following residues : -2)α-L-Rhap(1-->6)α-D-Glcp(1-->4)α-D-Galp [branched to β-D-GlcpNAc(1-->3)] (1-->3)α-D-GlcpNAc [branched to α-D-Glcp(1-->6)] (1-
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Boiled E. coli 018A bacteria
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab PL6
Antibody type and class	IgM
Assay System	ELISA, immunoblotting and Bacterial agglutination tests, competitive inhibition assays
Cross-reactivity	This Mab was specific to E. coli strain 018A and 018A1
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	24-01-2010
References	PMC261100

Entry No. - 2 [TOP]

PolysacDB ID	1081
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide part consists of the following residues : -2)α-L-Rhap(1-->6)α-D-Glcp(1-->4)α-D-Galp [branched to β-D-GlcpNAc(1-->3)] (1-->3)α-D-GlcpNAc [branched to α-D-Glcp(1-->6)] (1-
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Boiled E. coli 018A bacteria
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab PL4
Antibody type and class	IgM
Assay System	ELISA, immunoblotting and Bacterial agglutination tests, competitive inhibition assays
Cross-reactivity	This Mab was specific to E. coli strain 018A and 018A2 but cross-reacted with S. marcescens 08 LPS
Proposed epitopes	N-acetyl-D-glucosamine seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	24-01-2010
References	PMC261100

Entry No. - 3 [TOP]

PolysacDB ID	1082
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide part consists of the following residues : -2)α-L-Rhap(1-->6)α-D-Glcp(1-->4)α-D-Galp [branched to β-D-GlcpNAc(1-->3)] (1-->3)α-D-GlcpNAc [branched to α-D-Glcp(1-->6)] (1-
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Boiled E. coli 018A bacteria
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab PL5
Antibody type and class	IgM
Assay System	ELISA, immunoblotting and Bacterial agglutination tests, competitive inhibition assays
Cross-reactivity	This Mab was specific to E. coli strain 018A and 018A3
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	24-01-2010
References	PMC261100

Entry No. - 4 [TOP]

PolysacDB ID	1083
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide part consists of the following residues : -2)α-L-Rhap(1-->6)α-D-Glcp(1-->4)α-D-Galp [branched to β-D-GlcpNAc(1-->3)] (1-->3)α-D-GlcpNAc [branched to α-D-Glcp(1-->6)] (1-
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Boiled E. coli 018A bacteria
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab PL13
Antibody type and class	IgM
Assay System	ELISA, immunoblotting and Bacterial agglutination tests, competitive inhibition assays
Cross-reactivity	This Mab was specific to E. coli strain 018A and 018A2 but cross-reacted with S. marcescens 08 LPS
Proposed epitopes	N-acetyl-D-glucosamine seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	24-01-2010
References	PMC261100

Entry No. - 5 [TOP]

PolysacDB ID	1084
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide part consists of the following residues : -2)α-L-Rhap(1-->6)α-D-Glcp(1-->4)α-D-Galp [branched to β-D-GlcpNAc(1-->3)] (1-->3)α-D-GlcpNAc [branched to α-D-Glcp(1-->6)] (1-
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Boiled E. coli 018A bacteria
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab PL2
Antibody type and class	IgM
Assay System	ELISA, immunoblotting and Bacterial agglutination tests, competitive inhibition assays
Cross-reactivity	This Mab cross-reacted with E. coli strain O18B, O18B1 and O4.
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-01-2010
References	PMC261100

Entry No. - 6 [TOP]

PolysacDB ID	1085
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Escherichia coli O18A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-polysaccharide part consists of the following residues : -2)α-L-Rhap(1-->6)α-D-Glcp(1-->4)α-D-Galp [branched to β-D-GlcpNAc(1-->3)] (1-->3)α-D-GlcpNAc [branched to α-D-Glcp(1-->6)] (1-
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Boiled E. coli 018A bacteria
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab PL15
Antibody type and class	IgM
Assay System	ELISA, immunoblotting and Bacterial agglutination tests, competitive inhibition assays
Cross-reactivity	This Mab cross-reacted with E. coli strain O18B, O18B1, O23A and O4
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-01-2010
References	PMC261100

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