PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 3

Entry No. - 1   [TOP]
PolysacDB ID1450
Carbohydrate NameFVP2 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeFlammulina velutipes   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB StructureN/A
Proposed functionsFVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
AntibodiesMab B11D
Antibody type and classIgM
Assay SystemIndirect ELISA, Double Immunodiffusion
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator IDAA + AS
Date of Curation14-07-2010
References19108616


Entry No. - 2   [TOP]
PolysacDB ID1451
Carbohydrate NameFVP2 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeFlammulina velutipes   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB StructureN/A
Proposed functionsFVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
AntibodiesMab C8G
Antibody type and classIgM
Assay SystemIndirect ELISA, Double Immunodiffusion
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator IDAA + AS
Date of Curation14-07-2010
References19108616


Entry No. - 3   [TOP]
PolysacDB ID1452
Carbohydrate NameFVP2 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeFlammulina velutipes   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB StructureN/A
Proposed functionsFVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameBovine serum albumin
Conjugation MethodThe FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
AntibodiesMab I6D
Antibody type and classIgM
Assay SystemIndirect ELISA, Double Immunodiffusion
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator IDAA + AS
Date of Curation21-07-2010
References19108616


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