PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1915
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeGliocladium roseum   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesPurified cell wall fraction
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 6A5
Antibody type and classIgM
Assay SystemELISA, immunogold silver staining light microscopy, immunogold electron microscopy and immunoblottin
Cross-reactivityThis Mab specifically recognized G. roseum and closely related species such as G. catenulentum and Nectria cinnabarina and did not react with any of 21 sap-staining fungi tested
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used for studies of wood colonization by fungi and for investigations of the interactions between different fungi growing on wood
Curator IDAA + AS
Date of Curation28-11-2010
References1479354


Entry No. - 2   [TOP]
PolysacDB ID1916
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeGliocladium roseum   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesPurified cell wall fraction
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 3F12
Antibody type and classIgM
Assay SystemELISA, immunogold silver staining light microscopy, immunogold electron microscopy and immunoblottin
Cross-reactivityMonoclonal 3F12 recognized most of the biological control fungi tested and also showed reactivity with two of the 21 sap-staining fungi - Cladosporium cladosporioides and Phoma sp
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used for studies of wood colonization by fungi and for investigations of the interactions between different fungi growing on wood
Curator IDAA + AS
Date of Curation28-11-2010
References1479354


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