| PolysacDB ID | 1571 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Leptospira interrogans serovar pomona (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The oligosaccharide contained rhamnose, ribose, glucose, and glucosamine
|
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Diphtheria toxoid |
| Conjugation Method | The oligosaccharide was modified as follows : it was dissolved in N-ethylmorpholine-water-12 N HCl (2.5:6.5:1 [vol/vol/vol], pH 8.5) in concentrations of approximately 24 umol in 250ul. 125umol of N-succinimidyl S-acetylmercaptoacetate in 250ul of N,N dimethylacetamide was added to the oligosaccharide. This solution was added to modified diphtheria toxoid |
| Antibodies | Polysera |
| Antibody type and class | IgG and IgM |
| Assay System | Enzyme Immunoassay, immunoblotting and dot blot |
| Cross-reactivity | This antisera cross-reacted with LPS from some other leptospiral serovars (serovars hardjobovis, ballum, copenhageni, canicola, grippotyphosa, and batavia) and LPS from other bacteria (Salmonella typhimurium and the Re mutant of Shigella flexneri) and did not react with serovars australis, grippotyphosa, ballum, or tarassovi |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antiserum induced a high CL response in mouse macrophage monolayers |
| Curator ID | AA + AS |
| Date of Curation | 10-09-2010
|
| References | PMC303291 |