| PolysacDB ID | 1622 |
| Carbohydrate Name | Lipooligosaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Moraxella catarrhalis serotype C strain 26404 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to β-D-Galp-(1-->4)-α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-α-Kdop [branched to Kdop-(2-->4)]
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| BCSDB Structure | N/A |
| Proposed functions | Moracella lipooligosaccharide is a potential virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | 240 mg of lipooligosaccharide [LOS] was detoxified by using anhydrous hydrazine, and the dLOS was purified. Then adipic acid dihydrazide was conjugated to the dLOS (96 mg) in 12 ml of a 287 mM adipic acid dihydrazide suspension to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl and N-hydroxysulfosuccinimide. The resulting AH-dLOS was finally coupled to Tetanus toxoid [TT]. 10 mg of TT (Mr, 150,000) was reacted with 20 mg of AH-dLOS (10 mg/ml) at a molar ratio of AH-dLOS to TT of 100:1 using 0.05 M 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl. All reaction mixtures were maintained at pH 5.0 to 5.2 for 4 h at 4°C, and the reactions were stopped by adjusting the pH to 7.0. The reaction mixtures were dialyzed against 0.9% NaCl for 2 to 3 days, centrifuged, and passed through a Sephacryl S-300 column (2.6 by 90 cm) in 0.9% NaCl. Peaks that contained both protein and carbohydrate were pooled and designated dLOS-TT, dLOS-CRM-1, and dLOS-CRM-2. The three conjugates were analyzed to determine their carbohydrate and protein contents using dLOS and bovine serum albumin as standards |
| Antibodies | Polysera |
| Antibody type and class | N/A |
| Assay System | ELISA, Bactericidal assay and bactericidal inhibition assay |
| Cross-reactivity | This antisera cross-reacted with Serotype A LPS and to a little extent to Serotype B |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera showed complement-mediated bactericidal activity against the homologous strain |
| Curator ID | AA + AS |
| Date of Curation | 24-09-2010
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| References | PMC1932890 |