PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 27
Entry No. - 1 [TOP]

PolysacDB ID	1003
Carbohydrate Name	Glucan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
BCSDB Structure	N/A
Proposed functions	Activates leukocytes, stimulating their phagocytic, cytotoxic, and antimicrobial activities, including the production of reactive oxygen and nitrogen intermediates
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Pseudomonas aeruginosa exoprotein A (rEPA)
Conjugation Method	The polysaccharide was activated with CDAP. A 60-ml volume of CDAP (100 mg/ml of acetonitrile) was added to a solution of polysaccharide (2 ml, 10 mg of polysaccharide per ml of PFS) at room temperature. The pH was maintained at 5.8 to 6.0 for 30 s, and 60 ml of 0.2 M TEA was added to a pH of 7.0. The reaction was carried out for 2 min, and an equal volume of 0.8 M adipic acid dihydrazide [ADH] in 0.5 M NaHCO3 was added. This reaction was carried out for 2 h, and the pH was maintained at 8.0 to 8.5 with 0.1 N NaOH. The reaction mixture was dialyzed against PFS and passed through a column (3 by 46 cm) of P-10 in PFW. The void volume fractions were pooled, freeze-dried. ADH-derivatized polysaccharide (10 mg) was dissolved in PFS (2 ml). An equal weight of protein was added, and the pH was maintained at 5.1 to 5.5 with 0.1 M HCl. The reaction mixture was put on ice, EDAC was added to a final concentration of 0.05 M, and the pH was maintained at 5.1 to 5.5 for 4 h in 0.1 M HCl. The reaction mixtures were dialyzed against 0.2 M NaCl for 2 days with three changes of outer fluid and were passed through a column (1.5 by 90 cm) of Sepharose CL-6B in 0.2 M NaCl. The void volume fractions were stored at 3 to 88°C
Antibodies	Mab 24c5
Antibody type and class	IgG
Assay System	ELISA, Immunofluorescence
Cross-reactivity	This antibody cross-reacted with M. kansasii, BCG Pasteur, M. smegmatis, M. phlei, M. fortuitum, and M. avium
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	The antibody was used to study M. tuberculosis Glucan expression in vitro and in vivo
Curator ID	AA + AS
Date of Curation	01-01-2010
References	PMC127896

Entry No. - 2 [TOP]

PolysacDB ID	1007
Carbohydrate Name	Lipoarabinomannan (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Mycobacterial lipoarabinomannan is a phosphatidylinositol anchored lipoglycan that consists of an α1-->6 linked backbone, substituted to varying degrees at position 2 with single α-Man residues, and directly attached to position 6 of the myo-inositol of a phosphatidylinositol (PI) anchor
BCSDB Structure	127448
Proposed functions	T cell proliferation and to interfere with gamma interferon-mediated activation of macrophages
Antigenic Nature used to produce antibodies	Heat killed Mycobacterium tuberculosis
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 5c11
Antibody type and class	IgM
Assay System	Indirect immunofluorescence, ELISA, western blotting
Cross-reactivity	This antibody cross-reacted with large number of mycobacterial species.
Proposed epitopes	Arabinose moiety of Lipoarabinomannan
IEDB Epitope	N/A
Proposed Utility	This antibody has been used in the detection of mycobacteria in clinical samples
Curator ID	AA + AS
Date of Curation	02-01-2010
References	PMC229406

Entry No. - 3 [TOP]

PolysacDB ID	1008
Carbohydrate Name	Mycolyl-arabinogalactan-peptidoglycan complex (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Mycolyl-arabinogalactan-peptidoglycan complex is the fraction of the mycobacterial cell wall left after removing all soluble carbohydrates, proteins and lipids
BCSDB Structure	N/A
Proposed functions	Contains lipoarabinomannan as well as arabinose that are important virulence factors
Antigenic Nature used to produce antibodies	Heat killed Mycobacterium tuberculosis
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mabs 4f11
Antibody type and class	IgM
Assay System	Indirect immunofluorescence, ELISA, western blotting
Cross-reactivity	This antibody cross-reacted with large number of mycobacterial species.
Proposed epitopes	Arabinose moiety of Lipoarabinomannan
IEDB Epitope	N/A
Proposed Utility	This antibody has been used in the detection of mycobacteria in clinical samples
Curator ID	AA + AS
Date of Curation	02-01-2010
References	PMC229406

Entry No. - 4 [TOP]

PolysacDB ID	1009
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed Mycobacterium tuberculosis
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 9d8
Antibody type and class	IgG3
Assay System	Indirect immunofluorescence, ELISA, western blotting
Cross-reactivity	This antibody cross-reacted with M.gordonae, M. gastri, and M. Kansasii
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody has been used in the detection of mycobacteria in clinical samples
Curator ID	AA + AS
Date of Curation	03-01-2010
References	PMC229406

Entry No. - 5 [TOP]

PolysacDB ID	1010
Carbohydrate Name	Lipoarabinomannan (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Mycobacterial lipoarabinomannan is a phosphatidylinositol anchored lipoglycan that consists of an α1-->6 linked backbone, substituted to varying degrees at position 2 with single α-Man residues, and directly attached to position 6 of the myo-inositol of a phosphatidylinositol (PI) anchor
BCSDB Structure	127448
Proposed functions	Reported to suppress T cell proliferation and to interfere with gamma interferon-mediated activation of macrophages. Other reported effects include inhibition of protein kinases, inhibition of the synthesis of mRNA encoding IL-2, IL-5, and granulocyte-macrophage colony stimulating factor (GM-CSF) in human T cells and activation of the complement cascade
Antigenic Nature used to produce antibodies	Heat killed Mycobacterium tuberculosis
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	SMITB14
Antibody type and class	IgG1
Assay System	N\A
Cross-reactivity	SMITB14 bound to whole M. tuberculosis bacteria (H37Rv and Harlingen strains) as well as to the attenuated BCG, but not to other species such as Escherichia coli strains of various O serotypes, Streptococcus pneumoniae isolates, strains of Salmonella, Yersinia enterocolitica and Y. pseudotuberculosis and Nocardia sp.
Proposed epitopes	Arabinomannan portion of the antigen
IEDB Epitope	N/A
Proposed Utility	This antibody might be involved in blocking of mycobacterial invasion. SMITB14 may prevent the uptake of M. tuberculosis through receptor(s) that facilitate bacterial survival and growth.
Curator ID	AA + AS
Date of Curation	03-01-2010
References	PMC1809178

Entry No. - 6 [TOP]

PolysacDB ID	1011
Carbohydrate Name	Lipoarabinomannan (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Mycobacterial lipoarabinomannan is a phosphatidylinositol anchored lipoglycan that consists of an α1-->6 linked backbone, substituted to varying degrees at position 2 with single α-Man residues, and directly attached to position 6 of the myo-inositol of a phosphatidylinositol (PI) anchor
BCSDB Structure	127448
Proposed functions	Reported to suppress T cell proliferation and to interfere with gamma interferon-mediated activation of macrophages. Other reported effects include inhibition of protein kinases, inhibition of the synthesis of mRNA encoding IL-2, IL-5, and granulocyte-macrophage colony stimulating factor (GM-CSF) in human T cells and activation of the complement cascade
Antigenic Nature used to produce antibodies	Heat killed Mycobacterium tuberculosis
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab lam701
Antibody type and class	IgG3
Assay System	Sandwich ELISA
Cross-reactivity	This antibody cross-reacted with cross-reacted to a large number of mycobacterial species such as M. tuberculosis, M. bovis, M leprae, M. phlei and M. avium
Proposed epitopes	N\A
IEDB Epitope	N/A
Proposed Utility	These antibodies could be used to detect mycobacterial antigens in sputum
Curator ID	AA + AS
Date of Curation	03-01-2010
References	2127645

Entry No. - 7 [TOP]

PolysacDB ID	1627
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Unheated, lyophilized M. tuberculosis H37Ra culture filtrate solids
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB5-1
Antibody type and class	IgM
Assay System	Affinity chromatography, ELISA
Cross-reactivity	This Mab cross-reacted with M. kansasii
Proposed epitopes	Arabinomannan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-09-2010
References	PMC265954

Entry No. - 8 [TOP]

PolysacDB ID	1628
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Unheated, lyophilized M. tuberculosis H37Ra culture filtrate solids
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB5-2
Antibody type and class	IgG
Assay System	Affinity chromatography, ELISA
Cross-reactivity	This Mab cross-reacted with M. kansasii
Proposed epitopes	Arabinomannan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-09-2010
References	PMC265954

Entry No. - 9 [TOP]

PolysacDB ID	1629
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Unheated, lyophilized M. tuberculosis H37Ra culture filtrate solids
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB5-20
Antibody type and class	IgG2b
Assay System	Affinity chromatography, ELISA
Cross-reactivity	This Mab cross-reacted with M. kansasii
Proposed epitopes	Arabinomannan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-09-2010
References	PMC265954

Entry No. - 10 [TOP]

PolysacDB ID	1630
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Unheated, lyophilized M. tuberculosis H37Ra culture filtrate solids
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-1
Antibody type and class	IgM
Assay System	Affinity chromatography, ELISA
Cross-reactivity	This Mab cross-reacted with M. kansasii
Proposed epitopes	Arabinomannan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-09-2010
References	PMC265954

Entry No. - 11 [TOP]

PolysacDB ID	1744
Carbohydrate Name	Arabinogalactan and arabinomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Mycobacterial lipoarabinomannan is a phosphatidylinositol anchored lipoglycan that consists of an α1-->6 linked backbone, substituted to varying degrees at position 2 with single α-Man residues, and directly attached to position 6 of the myo-inositol of a phosphatidylinositol (PI) anchor
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-1
Antibody type and class	IgM
Assay System	ELISA, immunoblotting, Immunosorbent affinity chromatography and Immunoelectrophoresis
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare
Proposed epitopes	N/A
IEDB Epitope	76830
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	20-10-2010
References	PMC1577034

Entry No. - 12 [TOP]

PolysacDB ID	1746
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-1
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	20-10-2010
References	PMC265954

Entry No. - 13 [TOP]

PolysacDB ID	1747
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-2
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	21-10-2010
References	PMC265954

Entry No. - 14 [TOP]

PolysacDB ID	1748
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-3
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	21-10-2010
References	PMC265954

Entry No. - 15 [TOP]

PolysacDB ID	1749
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-4
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	21-10-2010
References	PMC265954

Entry No. - 16 [TOP]

PolysacDB ID	1750
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-5
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	21-10-2010
References	PMC265954

Entry No. - 17 [TOP]

PolysacDB ID	1751
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-6
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	22-10-2010
References	PMC265954

Entry No. - 18 [TOP]

PolysacDB ID	1752
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-7
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	22-10-2010
References	PMC265954

Entry No. - 19 [TOP]

PolysacDB ID	1753
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-8
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	22-10-2010
References	PMC265954

Entry No. - 20 [TOP]

PolysacDB ID	1754
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-9
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	23-10-2010
References	PMC265954

Entry No. - 21 [TOP]

PolysacDB ID	1755
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-10
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	23-10-2010
References	PMC265954

Entry No. - 22 [TOP]

PolysacDB ID	1756
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-11
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	23-10-2010
References	PMC265954

Entry No. - 23 [TOP]

PolysacDB ID	1757
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-12
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	23-10-2010
References	PMC265954

Entry No. - 24 [TOP]

PolysacDB ID	1758
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-16
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	23-10-2010
References	PMC265954

Entry No. - 25 [TOP]

PolysacDB ID	1759
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	M. tuberculosis culture filtrate
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-17
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	23-10-2010
References	PMC265954

Entry No. - 26 [TOP]

PolysacDB ID	1760
Carbohydrate Name	Antigen 5 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Antigen 5 is a well known 35,000-dalton glycoprotein of Mycobacteriun tuberculosis
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Purified M. tuberculosis Antigen 5
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab TB-C-19
Antibody type and class	IgM
Assay System	ELISA, immunoabsorbent affinity chromatography
Cross-reactivity	This Mab is highly cross-reactive and cross-reacted with M. kansasii, M. gordonae, M. scrofulaceum and M. intracellulare and also cross-reacted with antigen 6 from Mycobacterium tuberculosis
Proposed epitopes	Arabinomannan and arabinogalactan residues seemed to be a part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would allow the purification of important mycobacterial antigens antigens
Curator ID	AA + AS
Date of Curation	24-10-2010
References	PMC265954

Entry No. - 27 [TOP]

PolysacDB ID	1761
Carbohydrate Name	Peptide mimotope (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	The peptide consisted of the following sequence : QEPLMGTVPIRAGGGS
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Purified peptide
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Polysera
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	This antisera recognized mannosylated Mycobacterium tuberculosis cell-wall antigens arabinomannan, lipoarabinomannan and the glycosylated recombinant protein Apa. The antisera also cross-reacted with mannans from Saccharomyces cerevisiae, but did not react with phosphatidyl-inositol-di-mannoside or arabinogalactan from Mycobacteria
Proposed epitopes	The epitope was proposed to be oligomannosidic in nature
IEDB Epitope	N/A
Proposed Utility	This peptide mimotope can used as surrogate reagent for immunodiagnosis of tuberculosis
Curator ID	AA + AS
Date of Curation	24-10-2010
References	PMC1134969

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036