PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 3
Entry No. - 1 [TOP]

PolysacDB ID	1895
Carbohydrate Name	Glucosyl ceramide (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Paracoccidiodes brasiliensis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab MEST2
Antibody type and class	IgG2a
Assay System	High performance thin layer chromatography immunostaining, solid phase radioimmunoassay
Cross-reactivity	This Mab cross-reacts with glucosyl ceramide from Histoplasma capsulatum, Sporothrix schenckii, Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Cryptococcus laurentii and Cryptococcus albidus
Proposed epitopes	β-D-glucose residues and 2-hydroxyl groups in the fatty acid groups seemed to a important part of the epitoipe
IEDB Epitope	N/A
Proposed Utility	This Mab should act as an effective reagent in studies for the distribution of glycoinositol-phosphoryl ceramides in the plasma membrane of different fungi
Curator ID	AA + AS
Date of Curation	24-11-2010
References	11287397

Entry No. - 2 [TOP]

PolysacDB ID	1896
Carbohydrate Name	Band 1 glycolipid antigen (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe	Paracoccidiodes brasiliensis (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of glycolipid antigen is Galfβ1->6(Manpα1-->3)Manpβ1-->2Ins
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab MEST1
Antibody type and class	IgG3
Assay System	High performance thin layer chromatography immunostaining, solid phase radioimmunoassay
Cross-reactivity	This Mab cross-reacted with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi
Proposed epitopes	Terminal residue of β-D-galactofuranose of Band 1 seems to be a part of the epitope
IEDB Epitope	76842
Proposed Utility	MAb MEST-1 can be a valuable tool in studies of biological role of galactofuranose residues
Curator ID	AA + AS
Date of Curation	24-11-2010
References	9184826

Entry No. - 3 [TOP]

PolysacDB ID	1897
Carbohydrate Name	GP70 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Paracoccidiodes brasiliensis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Macerated polyacrylamide gel containing 70-kDa glycoprotein [gp70]
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab B7D6
Antibody type and class	IgG1
Assay System	ELISA, immunoblotting and inhibition assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be useful for immunodiagnostic studies
Curator ID	AA + AS
Date of Curation	24-11-2010
References	PMC219562

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036