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| PolysacDB ID | 2055 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Pseudomonas syringae IMV 7836 pv. Atrofaciens (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-] |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Ps1a |
| Antibody type and class | IgG3 |
| Assay System | Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting |
| Cross-reactivity | "All P. syringae strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi as well as 72 (87.8%), 3 (75%), and 31 (50%) strains with pathovars atrofaciens, striafaciens, and syringae, respectively, and 20 (80%) P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1a" |
| Proposed epitopes | The epitope of Mab 1a is related to the Rha(α1-->2)D-Rha(α1-->3)D-Rha sequence of the O-polysaccharide backbone with the immunodominant site (α1-->2)D-rhamnose(α1-->3) |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab was useful for elucidation of the LPs structure |
| Curator ID | AA + AS |
| Date of Curation | 31-12-2010 |
| References | PMC178531 |
| PolysacDB ID | 2056 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Pseudomonas syringae IMV 7836 pv. Atrofaciens (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-] |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Ps1a' |
| Antibody type and class | IgG3 |
| Assay System | Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting |
| Cross-reactivity | "Mab Ps1a' cross-reacted with all strains from subgroups 1b, 1c, and 1x but not with strains from 1d." |
| Proposed epitopes | The epitope of Mab1a' is related to the D-Rha(alpha1-3)D-Rha(alpha1-2)D-Rha site of the O-polysaccharide backbone consisting of tetra-D-rhamnose repeats. The residue (alpha1-3)DRha( alpha1-2) is immunodominant for epitope of Mab 1a' |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab was useful for elucidation of the LPs structure |
| Curator ID | AA + AS |
| Date of Curation | 31-12-2010 |
| References | PMC178531 |
| PolysacDB ID | 2057 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Pseudomonas syringae IMV 7836 pv. Atrofaciens (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-] |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab Ps1X |
| Antibody type and class | IgG1 |
| Assay System | Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting |
| Cross-reactivity | MAb Ps1x reacted with only the homologous strain IMV 7836 |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens |
| Curator ID | AA + AS |
| Date of Curation | 31-12-2010 |
| References | PMC178531 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |