PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 10

Entry No. - 1   [TOP]
PolysacDB ID2167
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesHeated, alcohol-acetone-extracted Sabnonella newport CDC 50 antigen
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 32-1-E3
Antibody type and classIgG3
Assay SystemEnzyme immunosorbent assay, immunoblotting
Cross-reactivityThis Mab cross-reacts with Sablonella groups C2 (0:6,8) and C3 (0:-,8) but not with those from any other serogroup tested
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe MAb agglutinated with all strains of Salmonella serogroups C2 and C3 tested but not with other bacteria. The MAb is useful as a replacement for absorbed, polyclonal, single-factor 0:8 antiserum to discriminate Salmonella serogroups C2 and C3 from serogroup Cl
Curator IDAA + AS
Date of Curation17-02-2011
ReferencesPMC270587


Entry No. - 2   [TOP]
PolysacDB ID2168
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab B1-1
Antibody type and classIgM
Assay SystemELISA, immunoblotting
Cross-reactivityThis Mab was specific to S. typhimurium serogroup B
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used as a serotyping reagent for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory
Curator IDAA + AS
Date of Curation17-02-2011
ReferencesPMC270232


Entry No. - 3   [TOP]
PolysacDB ID2169
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MO4-2
Antibody type and classIgG3
Assay SystemELISA, immunoblotting
Cross-reactivityThis Mab was specific to S. typhimurium serogroup B
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used as a serotyping reagent for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory
Curator IDAA + AS
Date of Curation18-02-2011
ReferencesPMC270232


Entry No. - 4   [TOP]
PolysacDB ID2170
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MO4-3
Antibody type and classIgG3
Assay SystemELISA, immunoblotting
Cross-reactivityThis Mab was also specific but cross-reacted with Salmonella paratyphi serogroup A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used as a serotyping reagent for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory
Curator IDAA + AS
Date of Curation19-02-2011
ReferencesPMC270232


Entry No. - 5   [TOP]
PolysacDB ID2171
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MO4-4
Antibody type and classIgG1
Assay SystemELISA, immunoblotting
Cross-reactivityThis Mab was specific to S. typhimurium serogroup B
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used as a serotyping reagent for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory
Curator IDAA + AS
Date of Curation19-02-2011
ReferencesPMC270232


Entry No. - 6   [TOP]
PolysacDB ID2172
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MO4-5
Antibody type and classIgG1
Assay SystemELISA, immunoblotting
Cross-reactivityThis Mab was specific to S. typhimurium serogroup B
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used as a serotyping reagent for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory
Curator IDAA + AS
Date of Curation21-02-2011
ReferencesPMC270232


Entry No. - 7   [TOP]
PolysacDB ID2173
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure124341
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MO4-8
Antibody type and classIgG3
Assay SystemELISA, immunoblotting
Cross-reactivityThis Mab was also specific but cross-reacted with Salmonella paratyphi serogroup A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used as a serotyping reagent for the identification of serogroup B salmonellae in a routine diagnostic bacteriology laboratory
Curator IDAA + AS
Date of Curation21-02-2011
ReferencesPMC270232


Entry No. - 8   [TOP]
PolysacDB ID2179
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure6380
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5A5
Antibody type and classIgM
Assay SystemELISA, immunoblotting, dot blot assay, immunoelectron microscopy
Cross-reactivityThis Mab reacted with S. typhimurium (LPS O:1, 4, 5, and 12) while failing to recognize S. typhimurium var. copenhagen (LPS O:1, 4, and 12). It reacted equally with live and heat-attenuated Salmonella B serovars containing LPS O-5 antigen
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to discriminate between S. typhimurium and S. typhimurium var. copenhagen
Curator IDAA + AS
Date of Curation23-02-2011
ReferencesPMC167767


Entry No. - 9   [TOP]
PolysacDB ID2180
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure6380
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5B2
Antibody type and classIgM
Assay SystemELISA, immunoblotting, dot blot assay, immunoelectron microscopy
Cross-reactivityThis Mab reacted with S. typhimurium (LPS O:1, 4, 5, and 12) while failing to recognize S. typhimurium var. copenhagen (LPS O:1, 4, and 12). It reacted equally with live and heat-attenuated Salmonella B serovars containing LPS O-5 antigen
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to discriminate between S. typhimurium and S. typhimurium var. copenhagen
Curator IDAA + AS
Date of Curation23-02-2011
ReferencesPMC167767


Entry No. - 10   [TOP]
PolysacDB ID2181
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSalmonella typhimurium   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The O-antigen part consists of the following residues : -2)-α-D-Manp [branched to α-Abep-(1-->3)] -(1-->4)-α-L-Rhap-(1-->3)-α-D-Galp-(1-
BCSDB Structure6380
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 4A8
Antibody type and classIgG2a
Assay SystemELISA, immunoblotting, dot blot assay, immunoelectron microscopy
Cross-reactivityThis Mab reacted with S. typhimurium (LPS O:1, 4, 5, and 12) while failing to recognize S. typhimurium var. copenhagen (LPS O:1, 4, and 12). It reacted equally with live and heat-attenuated Salmonella B serovars containing LPS O-5 antigen
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to discriminate between S. typhimurium and S. typhimurium var. copenhagen
Curator IDAA + AS
Date of Curation24-02-2011
ReferencesPMC167767


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