PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 8
Entry No. - 1 [TOP]

PolysacDB ID	2339
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco1
Antibody type and class	IgM
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	06-04-2011
References	7686001

Entry No. - 2 [TOP]

PolysacDB ID	2340
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco2
Antibody type and class	IgG2b
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab cross-reacted with the closely related species S. benthica
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	06-04-2011
References	7686001

Entry No. - 3 [TOP]

PolysacDB ID	2341
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco3
Antibody type and class	IgG2a
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab cross-reacted with the closely related species S. benthica
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Entry No. - 4 [TOP]

PolysacDB ID	2342
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco4
Antibody type and class	IgG2b
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Entry No. - 5 [TOP]

PolysacDB ID	2343
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco5
Antibody type and class	IgG2a
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Entry No. - 6 [TOP]

PolysacDB ID	2344
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco6
Antibody type and class	IgG1
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Entry No. - 7 [TOP]

PolysacDB ID	2345
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco22
Antibody type and class	IgG3
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	09-04-2011
References	7686001

Entry No. - 8 [TOP]

PolysacDB ID	2346
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab Aco23
Antibody type and class	IgG1
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed epitopes	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	09-04-2011
References	7686001

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036