PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID2349
Carbohydrate NameK antigens [Capsular polysaccharide]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeSinorhizobium meliloti strain NRG185   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe K antigens of Sinorhizobium spp. are major strain-specific antigens, which commonly consist of small repeating units of a hexose and 1-carboxy-2-keto-3-deoxy sugars, such as sialic acid or 3-deoxy-D-manno-2-octulosonic acid (Kdo), although they vary in glycosyl residue composition, substitution patterns, linkage points, anomeric configuration, and size range. The K-antigens of rhizobia do not possess a lipid anchor
BCSDB StructureN/A
Proposed functionsK antigens may be involved in an early recognition step in S. meliloti-alfalfa interactions and that K antigen production is affected by plant-derived signals
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 8
Antibody type and classIgGM
Assay SystemELISA and immunoblotting
Cross-reactivityThis Mab was specific to S. melliloti. But MAb 8 recognized the K antigens from both the homologous strain, S. meliloti NRG185, and S. meliloti NRG286
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development
Curator IDAA + AS
Date of Curation11-04-2011
References10543844


Entry No. - 2   [TOP]
PolysacDB ID2352
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeSinorhizobium meliloti strain NRG185   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThere are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
BCSDB StructureN/A
Proposed functionsLPS is an important surface antigen
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 11
Antibody type and classIgM
Assay SystemELISA and immunoblotting
Cross-reactivityThis Mab was highly cross-reactive recognizing most number of Shinorhizobium species. MAb 11 recognized only one major form (band) of R-LPS from the serogroup A strains, and it did not bind the S-LPS from any strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development
Curator IDAA + AS
Date of Curation12-04-2011
References10543844


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