PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 31

Entry No. - 1   [TOP]
PolysacDB ID2355
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-1
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation14-04-2011
References4008045


Entry No. - 2   [TOP]
PolysacDB ID2356
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-2
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation14-04-2011
References4008045


Entry No. - 3   [TOP]
PolysacDB ID2357
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-4
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation14-04-2011
References4008045


Entry No. - 4   [TOP]
PolysacDB ID2358
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-5
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation16-04-2011
References4008045


Entry No. - 5   [TOP]
PolysacDB ID2359
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-6
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation16-04-2011
References4008045


Entry No. - 6   [TOP]
PolysacDB ID2360
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-7
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation16-04-2011
References4008045


Entry No. - 7   [TOP]
PolysacDB ID2361
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-8
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation16-04-2011
References4008045


Entry No. - 8   [TOP]
PolysacDB ID2362
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-10
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation16-04-2011
References4008045


Entry No. - 9   [TOP]
PolysacDB ID2363
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-11
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation16-04-2011
References4008045


Entry No. - 10   [TOP]
PolysacDB ID2364
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-12
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation17-04-2011
References4008045


Entry No. - 11   [TOP]
PolysacDB ID2365
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-13
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation17-04-2011
References4008045


Entry No. - 12   [TOP]
PolysacDB ID2366
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-14
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation17-04-2011
References4008045


Entry No. - 13   [TOP]
PolysacDB ID2367
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-17
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation17-04-2011
References4008045


Entry No. - 14   [TOP]
PolysacDB ID2368
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-18
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation18-04-2011
References4008045


Entry No. - 15   [TOP]
PolysacDB ID2369
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-20
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation19-04-2011
References4008045


Entry No. - 16   [TOP]
PolysacDB ID2370
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-21
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation19-04-2011
References4008045


Entry No. - 17   [TOP]
PolysacDB ID2371
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-22
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation19-04-2011
References4008045


Entry No. - 18   [TOP]
PolysacDB ID2372
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-23
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation20-04-2011
References4008045


Entry No. - 19   [TOP]
PolysacDB ID2373
Carbohydrate NameType 5 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 17-24
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of four different type 5 S. aureus clinical isolates but with none of the five S. aureus clinical isolates possessing type 8 capsular polysaccharides. This Mab was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 6, and 7, but was reactive with type 4 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation21-04-2011
References4008045


Entry No. - 20   [TOP]
PolysacDB ID2374
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-1
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation21-04-2011
References4008045


Entry No. - 21   [TOP]
PolysacDB ID2375
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-2
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation21-04-2011
References4008045


Entry No. - 22   [TOP]
PolysacDB ID2376
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-4
Antibody type and classIgM
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation21-04-2011
References4008045


Entry No. - 23   [TOP]
PolysacDB ID2377
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-5
Antibody type and classIgG3
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation22-04-2011
References4008045


Entry No. - 24   [TOP]
PolysacDB ID2378
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-8
Antibody type and classIgG3
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation22-04-2011
References4008045


Entry No. - 25   [TOP]
PolysacDB ID2379
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-9
Antibody type and classIgG3
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation23-04-2011
References4008045


Entry No. - 26   [TOP]
PolysacDB ID2380
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-10
Antibody type and classIgG3
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation23-04-2011
References4008045


Entry No. - 27   [TOP]
PolysacDB ID2381
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-11
Antibody type and classIgG3
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation23-04-2011
References4008045


Entry No. - 28   [TOP]
PolysacDB ID2382
Carbohydrate NameType 8 Capsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructureType 5 capsular polysaccharide consists of following residues : -4)-β-D-ManpNAcA3Ac-(1-->4)-α-L-FucpNAc-(1-->3)-β-D-FucpNAc-(1-
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalinized cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 18-12
Antibody type and classIgG3
Assay SystemELISA and Bacterial agglutination assays
Cross-reactivityThis Mab reacted with each of five type 8 S. aureus strains but not with any type 5 strains. It was also completely unreactive with S. aureus strains possessing capsular types 1, 2, 4, and 7, but reacted with type 6 strain
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will be used in various studies of S. aureus capsular polysaccharides. In particular, the use of these antibodies should facilitate the biochemical purification and further characterization of these polysaccharides. In addition, these reagents can be used to investigate the role of capsular polysaccharides in the invasiveness and virulence of encapsulated strains of S. aureus. Knowledge gained from these studies will be applicable to the development of a suitable vaccine. These antibodies will also be evaluated as diagnostic reagents for use in staphylococcal disease
Curator IDAA + AS
Date of Curation24-04-2011
References4008045


Entry No. - 29   [TOP]
PolysacDB ID2383
Carbohydrate NameSurface Polysaccharide Poly-N-Acetylglucosamine [PNAG]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructurePNAG consists of a backbone of N-acetylglucosamine residues of variable lendth
BCSDB Structure21574
Proposed functionsPNAG also referred to as the polysaccharide intercellular adhesin, has been shown to elicit opsonic antibodies when used as a vaccine in goats and rabbits. In addition, these polyclonal antibodies passively protect mice against S. aureus bacteremia and renal infection as well as against lethality following a high-dose infection. Animal antibodies to PNAG also mediate killing of S. epidermidis strains that express this antigen, and these strains constitute a significant proportion of clinical isolates
Antigenic Nature used to produce antibodiesStaphylococcus aureus infected patients
Carrier NameNil
Conjugation MethodNil
AntibodiesMab F598
Antibody type and classIgG1
Assay SystemEnzyme-linked immunosorbent assays, complement deposition, immunofluorescence and opsonophagocytic assays
Cross-reactivityThis Mab was specfic to S. aureus and was found to be nonreactive against alginate from Pseudomonas aeruginosa strain PAO 581, the P. aeruginosa LPS O antigens from serogroups 02 and 06, and the PS/A antigen of Bacteroides fragilis
Proposed epitopesThis Mab bound best to nonacetylated or backbone epitopes on PNAG
IEDB EpitopeN/A
Proposed UtilityThis Mab had superior complement deposition and opsonophagocytic activity. This Mab also had optimal protective efficacy. This Mab could be immunotherapeutic agent for preventing or treating staphylococcal infections.
Curator IDAA + AS
Date of Curation24-04-2011
References16622211


Entry No. - 30   [TOP]
PolysacDB ID2384
Carbohydrate NameSurface Polysaccharide Poly-N-Acetylglucosamine [PNAG]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructurePNAG consists of a backbone of N-acetylglucosamine residues of variable lendth
BCSDB Structure21574
Proposed functionsPNAG also referred to as the polysaccharide intercellular adhesin, has been shown to elicit opsonic antibodies when used as a vaccine in goats and rabbits. In addition, these polyclonal antibodies passively protect mice against S. aureus bacteremia and renal infection as well as against lethality following a high-dose infection. Animal antibodies to PNAG also mediate killing of S. epidermidis strains that express this antigen, and these strains constitute a significant proportion of clinical isolates
Antigenic Nature used to produce antibodiesStaphylococcus aureus infected patients
Carrier NameNil
Conjugation MethodNil
AntibodiesMab F628
Antibody type and classIgG1
Assay SystemEnzyme-linked immunosorbent assays, complement deposition, immunofluorescence and opsonophagocytic assays
Cross-reactivityThis Mab was specfic to S. aureus and was found to be nonreactive against alginate from Pseudomonas aeruginosa strain PAO 581, the P. aeruginosa LPS O antigens from serogroups 02 and 06, and the PS/A antigen of Bacteroides fragilis
Proposed epitopesThis Mab bound best to PNAG containing N-acetyl groups
IEDB EpitopeN/A
Proposed UtilityThis Mab had partial complement deposition and opsonophagocytic activity.
Curator IDAA + AS
Date of Curation24-04-2011
References16622211


Entry No. - 31   [TOP]
PolysacDB ID2385
Carbohydrate NameSurface Polysaccharide Poly-N-Acetylglucosamine [PNAG]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStaphylococcus aureus   (NCBI Taxonomy)   (Drugpedia)
Basic StructurePNAG consists of a backbone of N-acetylglucosamine residues of variable lendth
BCSDB Structure21574
Proposed functionsPNAG also referred to as the polysaccharide intercellular adhesin, has been shown to elicit opsonic antibodies when used as a vaccine in goats and rabbits. In addition, these polyclonal antibodies passively protect mice against S. aureus bacteremia and renal infection as well as against lethality following a high-dose infection. Animal antibodies to PNAG also mediate killing of S. epidermidis strains that express this antigen, and these strains constitute a significant proportion of clinical isolates
Antigenic Nature used to produce antibodiesStaphylococcus aureus infected patients
Carrier NameNil
Conjugation MethodNil
AntibodiesMab F630
Antibody type and classIgG1
Assay SystemEnzyme-linked immunosorbent assays, complement deposition, immunofluorescence and opsonophagocytic assays
Cross-reactivityThis Mab was specfic to S. aureus and was found to be nonreactive against alginate from Pseudomonas aeruginosa strain PAO 581, the P. aeruginosa LPS O antigens from serogroups 02 and 06, and the PS/A antigen of Bacteroides fragilis
Proposed epitopesThis Mab bound best to PNAG containing N-acetyl groups
IEDB EpitopeN/A
Proposed UtilityThis Mab had partial complement deposition and opsonophagocytic activity.
Curator IDAA + AS
Date of Curation24-04-2011
References16622211


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036