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| PolysacDB ID | 1012 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1- |
| BCSDB Structure | 6237 |
| Proposed functions | Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8°C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Polysera |
| Antibody type and class | Mainly IgG, particularly IgG1 and IgG3 |
| Assay System | Double immunodiffusion, capillary precipitation, ELISA |
| Cross-reactivity | N/A |
| Proposed epitopes | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC258520 |
| PolysacDB ID | 2410 |
| Carbohydrate Name | Type 1b polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | A mixture composed of equal parts of the vaccine suspensions of strains H36B (type Tb), 18RS21 (type II), and 1073 (nontypeable) |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab SMB19 |
| Antibody type and class | IgM |
| Assay System | ELISA, indirect immunofluorescence |
| Cross-reactivity | This Mab reacted specifically with the sialylated form of the type 1b polysaccharide. This Mab cross-reacts with some oligosaccharides in human milk and certain fetal antigens |
| Proposed epitopes | Sialyllacto-N-tetraose residues seemed to be the immunodominant epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab will help in the development of appropriate vaccines against GBS that would include the possibility of inducing major cross-reactions with human glycoconjugates |
| Curator ID | AA + AS |
| Date of Curation | 02-05-2011 |
| References | 1548081 |
| PolysacDB ID | 2411 |
| Carbohydrate Name | Type 1b polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | H36B vaccine that had been treated with 2 N acetic acid at 80°C for 10 min to largely desialylate the type lb polysaccharide. |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab SIbD2 |
| Antibody type and class | IgM |
| Assay System | ELISA, indirect immunofluorescence |
| Cross-reactivity | This Mab reacted specifically with the de-sialylated form of the type 1b polysaccharide. This Mab cross-reacts with some oligosaccharides in human milk and certain fetal antigens |
| Proposed epitopes | Lacto-N-tetraose residues seemed to be the immunodominant epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab will help in the development of appropriate vaccines against GBS that would include the possibility of inducing major cross-reactions with human glycoconjugates |
| Curator ID | AA + AS |
| Date of Curation | 03-05-2011 |
| References | 1548081 |
| PolysacDB ID | 2413 |
| Carbohydrate Name | Type V capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The native type v polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-glucose and sialic acid in a molar ratio of 3:2:1:1. It consists of a repeating backbone of -->4)-α-D-Glcp-(1-->4)-α-D-Galp-(1-->4)-β-D-Glcp-(1--> with branching at 6th and 3rd positions |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Antisera |
| Antibody type and class | N/A |
| Assay System | ELISA, Ouchterlony diffusion |
| Cross-reactivity | Antibodies against the typeV capsuled o not cross-reactw ith other GBS capsular types and appear to recognize an epitope which is not as heavily dependent on the presenceo f the terminal side chain sialic acid residues as that of the type Ia, 11, and I11 GBS polysaccharides |
| Proposed epitopes | β-D-Gal-(1-->4)-β-D-GlcNAc segmenot of the trisaccharide side chain, which is present in the core but not in the backbone polysaccharide, also contributes to the native epitope. The residues of the linear backbone and/or the glucopyranosyl side chain also form part of the antibody-binding epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera helped in the determination of immunodominant epitopes in Type V polysaccharide in group B Streptococcus |
| Curator ID | AA + AS |
| Date of Curation | 04-05-2011 |
| References | 2016287 |
| PolysacDB ID | 2422 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site |
| BCSDB Structure | N/A |
| Proposed functions | The property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugation Method | Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C. Adipic acid dihydrazide was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The CP-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and TT per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column |
| Antibodies | Antisera |
| Antibody type and class | IgG |
| Assay System | Double immunodiffusion and capillary precipitation, opsonization assays |
| Cross-reactivity | This antisera was quite specific and did not react with the structurally related pneumococcus type 14 polysaccharide |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Conjugate-induced antibodies facilitated opsonization of group B streptococcus type III organisms. The type III-TT conjugate can be a potential vaccine for prevention of neonatal Group B Streptococcal diseases. |
| Curator ID | AA + AS |
| Date of Curation | 08-05-2011 |
| References | 2407652 |
| PolysacDB ID | 2423 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site |
| BCSDB Structure | N/A |
| Proposed functions | The property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab GBS II/4-1OBA9 |
| Antibody type and class | N/A |
| Assay System | Counterimmunoelectrophoresis, Radioactive binding assay, Ouchterlony double-diffusion, Latex particle agglutination tests |
| Cross-reactivity | A9 antibody cross-reacted with group B antigen in gel diffusion reactions with extracts of Lancefield group A, B, C, D, F, and G streptococci |
| Proposed epitopes | Rhamnose is not an immunodominant determinant contrasts with polyclonal rabbit antiserum to this antigen |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab was a highly sensitive reagent in detecting soluble group B antigen by counterimmunoelectrophoresis. A9 antibody-coated latex particles were compared with a commercially available polyclonal latex agglutination reagent and shown to be equally sensitive and specific in the detection of soluble group B antigen in urine and cerebrospinal fluid from patients with GBS infections |
| Curator ID | AA + AS |
| Date of Curation | 08-05-2011 |
| References | PMC272311 |
| PolysacDB ID | 2424 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site |
| BCSDB Structure | N/A |
| Proposed functions | The property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide |
| Antigenic Nature used to produce antibodies | Formalin treated cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab GBS III/3-5AC8 |
| Antibody type and class | N/A |
| Assay System | Counterimmunoelectrophoresis, Radioactive binding assay, Ouchterlony double-diffusion, Latex particle agglutination tests |
| Cross-reactivity | C8 antibody cross-reacted with group B antigen in gel diffusion reactions with extracts of Lancefield group A, B, C, D, F, and G streptococci |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | N/A |
| Curator ID | AA + AS |
| Date of Curation | 08-05-2011 |
| References | PMC272311 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |