| PolysacDB ID | 2435 |
| Carbohydrate Name | Trisaccharide part of Streptococcus pneumoniae type 3 polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Streptococcus pneumoniae Type 3 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A
|
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Diphtheria toxin mutant (CRM197) |
| Conjugation Method | The saccharide fragments described above were conjugated to CRM197 using diethyl squarate as linker. Elongation of the saccharides with diethyl squarate was performed in ethanol/0.1M sodium phosphate (pH 6.9). The reaction products were purified by solid-phase extraction and coupled to protein in 0.1m sodium borate buffer at pH 9.5 |
| Antibodies | Antisera |
| Antibody type and class | N/A |
| Assay System | Enzyme-linked immunosorbent assay and in-vitro phagocytosis assay |
| Cross-reactivity | N/A |
| Proposed epitopes | β-d-Glcp-(1-->3)-β-d-GlcpA-(1-->4)-β-d-Glcp |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera provided full protection against challenge with pneumococcal type 3 bacteria |
| Curator ID | AA + AS |
| Date of Curation | 13-05-2011
|
| References | PMC308892 |