PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 7
Entry No. - 1 [TOP]

PolysacDB ID	2427
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab B2
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit and human erythrocytes at temperatures below 37°C. This Mab also reacted with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit and human erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	10-05-2011
References	3989304

Entry No. - 2 [TOP]

PolysacDB ID	2428
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab F5
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit erythrocytes at temperatures below 37°C, but did not react with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	11-05-2011
References	3989304

Entry No. - 3 [TOP]

PolysacDB ID	2429
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab F9
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit and human erythrocytes at temperatures below 37°C. This Mab also reacted with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit and human erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	11-05-2011
References	3989304

Entry No. - 4 [TOP]

PolysacDB ID	2430
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 4AC10
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit and human erythrocytes at temperatures below 37°C. This Mab also reacted with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit and human erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	11-05-2011
References	3989304

Entry No. - 5 [TOP]

PolysacDB ID	2431
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 4AC5
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit and human erythrocytes at temperatures below 37°C. This Mab also reacted with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit and human erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	11-05-2011
References	3989304

Entry No. - 6 [TOP]

PolysacDB ID	2432
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 4BF5
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit and human erythrocytes at temperatures below 37°C. This Mab also reacted with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit and human erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	12-05-2011
References	3989304

Entry No. - 7 [TOP]

PolysacDB ID	2433
Carbohydrate Name	Streptococcus pneumoniae type XIV polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae type XIV (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 5DB4
Antibody type and class	IgM
Assay System	Hemagglutination, ELISA, Cryoprecipitation and cryoprecipitation-inhibition assays
Cross-reactivity	This Mab cross-reacted with rabbit and human erythrocytes at temperatures below 37°C. This Mab also reacted with phenylLac-BGG
Proposed epitopes	This Mab was specific for N-acetyl-lactosamine, the immunodominant sugar residue expressed on type XIV polysaccharide
IEDB Epitope	N/A
Proposed Utility	This Mab precipitated at low temperatures and also reacted with rabbit and human erythrocytes at low temperature
Curator ID	AA + AS
Date of Curation	12-05-2011
References	3989304

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036