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| PolysacDB ID | 2500 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae 01 Inaba 569B strain (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | N/A |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab F11P411F |
| Antibody type and class | IgG1 |
| Assay System | Enzyme-linked immunosorbent assay (ELISA), immunoblotting and fluorescein isothiocyanate (FITC) immunofluorescence tests |
| Cross-reactivity | This Mab cross-reacted with LPS extracted from the same species, including V. alginolyticus, V. anguillarum, V. cholerae 01, V. firnissii, V. ordalii, and V. parahaemolyticus, but did not cross-react with other species within the family Vibrionaceae (i.e., Aeromonas, Photobacterinum, and Plesiomonas species) or other gram-negative bacteria |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The use of the Mabs can be in blind tests and diagnosis of clinical isolates |
| Curator ID | AA + AS |
| Date of Curation | 05-06-2011 |
| References | PMC183162 |
| PolysacDB ID | 2501 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae 01 Inaba 569B strain (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | N/A |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab F7P52C |
| Antibody type and class | IgG2a |
| Assay System | Enzyme-linked immunosorbent assay (ELISA), immunoblotting and fluorescein isothiocyanate (FITC) immunofluorescence tests |
| Cross-reactivity | This Mab cross-reacted with LPS extracted from the same species, including V. alginolyticus, V. anguillarum, V. cholerae 01, V. firnissii, V. ordalii, and V. parahaemolyticus, but did not cross-react with other species within the family Vibrionaceae (i.e., Aeromonas, Photobacterinum, and Plesiomonas species) or other gram-negative bacteria |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | The use of the Mabs can be in blind tests and diagnosis of clinical isolates |
| Curator ID | AA + AS |
| Date of Curation | 07-06-2011 |
| References | PMC183162 |
| PolysacDB ID | 2553 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae 01 Inaba 569B strain (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | N/A |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Purified LPS |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab C6 |
| Antibody type and class | IgG3 |
| Assay System | Enzyme-linked immunosorbent assay inhibition experiments |
| Cross-reactivity | This Mab was specific to Inaba 35 and 569B LPS only |
| Proposed epitopes | This Mab was specific to C antigen |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab had a good agglutinating capacity when tested against V. cholerae 0:1 strains |
| Curator ID | AA + AS |
| Date of Curation | 25-06-2011 |
| References | PMC270838 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |