PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 19
Entry No. - 1 [TOP]

PolysacDB ID	2514
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 1
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab showed almost identical reactivities to both the Ogawa- and the Inaba-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant
Curator ID	AA + AS
Date of Curation	13-06-2011
References	PMC266894

Entry No. - 2 [TOP]

PolysacDB ID	2515
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 2
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab showed almost identical reactivities to both the Ogawa- and the Inaba-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant
Curator ID	AA + AS
Date of Curation	13-06-2011
References	PMC266894

Entry No. - 3 [TOP]

PolysacDB ID	2516
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 3
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab showed almost identical reactivities to both the Ogawa- and the Inaba-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant
Curator ID	AA + AS
Date of Curation	14-06-2011
References	PMC266894

Entry No. - 4 [TOP]

PolysacDB ID	2517
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 4
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab showed almost identical reactivities to both the Ogawa- and the Inaba-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant
Curator ID	AA + AS
Date of Curation	15-06-2011
References	PMC266894

Entry No. - 5 [TOP]

PolysacDB ID	2518
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 5
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab showed almost identical reactivities to both the Ogawa- and the Inaba-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant
Curator ID	AA + AS
Date of Curation	15-06-2011
References	PMC266894

Entry No. - 6 [TOP]

PolysacDB ID	2519
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 6
Antibody type and class	IgG2b
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab showed almost identical reactivities to both the Ogawa- and the Inaba-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant
Curator ID	AA + AS
Date of Curation	15-06-2011
References	PMC266894

Entry No. - 7 [TOP]

PolysacDB ID	2520
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 7
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted to Inaba-type cells but only very weakly reacted to Ogawa-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant. Moreover, highly Inaba specific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	16-06-2011
References	PMC266894

Entry No. - 8 [TOP]

PolysacDB ID	2521
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 8
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted to Inaba-type cells but only very weakly reacted to Ogawa-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant. Moreover, highly Inaba specific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	16-06-2011
References	PMC266894

Entry No. - 9 [TOP]

PolysacDB ID	2522
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 9
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted to Inaba-type cells but only very weakly reacted to Ogawa-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant. Moreover, highly Inaba specific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	16-06-2011
References	PMC266894

Entry No. - 10 [TOP]

PolysacDB ID	2523
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 10
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted to Inaba-type cells but only very weakly reacted to Ogawa-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant. Moreover, highly Inaba specific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	16-06-2011
References	PMC266894

Entry No. - 11 [TOP]

PolysacDB ID	2524
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 11
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted to Inaba-type cells but only very weakly reacted to Ogawa-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant. Moreover, highly Inaba specific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	16-06-2011
References	PMC266894

Entry No. - 12 [TOP]

PolysacDB ID	2525
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 12
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted to Inaba-type cells but only very weakly reacted to Ogawa-type cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant. Moreover, highly Inaba specific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	16-06-2011
References	PMC266894

Entry No. - 13 [TOP]

PolysacDB ID	2526
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 13
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab did not show cross-reactivity to Brucella abortus and Yersinia enterocolitica 09
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	17-06-2011
References	PMC266894

Entry No. - 14 [TOP]

PolysacDB ID	2527
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 14
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus and Yersinia enterocolitica 09
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	17-06-2011
References	PMC266894

Entry No. - 15 [TOP]

PolysacDB ID	2528
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 15
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	17-06-2011
References	PMC266894

Entry No. - 16 [TOP]

PolysacDB ID	2529
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 16
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus and Yersinia enterocolitica 11
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	17-06-2011
References	PMC266894

Entry No. - 17 [TOP]

PolysacDB ID	2530
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 17
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	18-06-2011
References	PMC266894

Entry No. - 18 [TOP]

PolysacDB ID	2531
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 18
Antibody type and class	IgG3
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	18-06-2011
References	PMC266894

Entry No. - 19 [TOP]

PolysacDB ID	2532
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae 01 serogroup Inaba strain X19203 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	N/A
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab IH 19
Antibody type and class	IgG1
Assay System	Slide agglutination, microdilution agglutination, and passive hemagglutination tests
Cross-reactivity	This Mab reacted only to Inaba-type cells; no agglutination was observed for Ogawa-type cells in either the slide agglutination or the microdilution agglutination tests. This Mab showed cross-reactivity to Brucella abortus and Yersinia enterocolitica 14
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab showed strong agglutination with both live cells and heat-killed cells. This Mab will be a very useful tool for resolution of the antigenic structure of V. cholerae 01, especially the Inaba-specific determinant, as well as crossreactive structure to B. abortus. Moreover, highly Inabaspecific antibodies will be able to be used in the identification of Inaba serotypes instead of polyclonal Inaba-specific antisera
Curator ID	AA + AS
Date of Curation	19-06-2011
References	PMC266894

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036