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| PolysacDB ID | 2496 |
| Carbohydrate Name | O-polysaccharide [Terminal disaccharide] (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 6642 |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
| Antibodies | Antisera |
| Antibody type and class | IgM and IgG1 |
| Assay System | ELISA |
| Cross-reactivity | This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76775 |
| Proposed Utility | This antisera was neither vibriocidal nor protective in the infant mouse cholera model |
| Curator ID | AA + AS |
| Date of Curation | 05-06-2011 |
| References | PMC427411 |
| PolysacDB ID | 2497 |
| Carbohydrate Name | O-polysaccharide [Terminal trisaccharide] (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 6642 |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
| Antibodies | Antisera |
| Antibody type and class | IgM and IgG2 |
| Assay System | ELISA |
| Cross-reactivity | This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76775 |
| Proposed Utility | N/A |
| Curator ID | AA + AS |
| Date of Curation | 05-06-2011 |
| References | PMC427411 |
| PolysacDB ID | 2498 |
| Carbohydrate Name | O-polysaccharide [Terminal tetrasaccharide] (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 6642 |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
| Antibodies | Antisera |
| Antibody type and class | IgM and IgG3 |
| Assay System | ELISA, invivo protection assay |
| Cross-reactivity | This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS |
| Proposed epitopes | N/A |
| IEDB Epitope | 76775 |
| Proposed Utility | This antisera helped in the epitope studies of O1 LPS |
| Curator ID | AA + AS |
| Date of Curation | 05-06-2011 |
| References | PMC427411 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |