| PolysacDB ID | 2495 |
| Carbohydrate Name | O-polysaccharide [Terminal hexasaccharides] (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae O1 LPS serotype ogawa (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar is characterized by a 2-O-methyl group
|
| BCSDB Structure | 6642 |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugation Method | The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates |
| Antibodies | Antisera |
| Antibody type and class | IgG and IgM |
| Assay System | ELISA |
| Cross-reactivity | N/A |
| Proposed epitopes | The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain |
| IEDB Epitope | 76824 |
| Proposed Utility | This antisera showed protection |
| Curator ID | AA + AS |
| Date of Curation | 04-06-2011
|
| References | 11920320 |