PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 8

Entry No. - 1   [TOP]
PolysacDB ID2564
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 2E5
Antibody type and classIgM
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. Mab 2E5 recognized only S-LPS and did not recognize R-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation30-06-2011
References10473431


Entry No. - 2   [TOP]
PolysacDB ID2565
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 1B10
Antibody type and classIgG1
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation01-07-2011
References10473431


Entry No. - 3   [TOP]
PolysacDB ID2566
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 3E10
Antibody type and classIgG3
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation01-07-2011
References10473431


Entry No. - 4   [TOP]
PolysacDB ID2567
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 6A1
Antibody type and classIgG1
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation02-07-2011
References10473431


Entry No. - 5   [TOP]
PolysacDB ID2568
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 6A12
Antibody type and classIgG2b
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation02-07-2011
References10473431


Entry No. - 6   [TOP]
PolysacDB ID2569
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 9H3
Antibody type and classIgG1
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation02-07-2011
References10473431


Entry No. - 7   [TOP]
PolysacDB ID2570
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 12G7
Antibody type and classIgG3
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation02-07-2011
References10473431


Entry No. - 8   [TOP]
PolysacDB ID2571
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeXanthomonas hyacinthi S148   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCrude cell wall preparation and purified fimbriae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 2D10
Antibody type and classIgG1
Assay SystemImmunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivityThis Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopesThe O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. This Mab recognized both R-LPS and S-LPS
IEDB EpitopeN/A
Proposed UtilityThis Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
Curator IDAA + AS
Date of Curation02-07-2011
References10473431


Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036