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| PolysacDB ID | 1185 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-1 |
| Antibody type and class | IgG2b |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab agglutinated bacteria and had high ELISA titers, but which failed to precipitate the polysaccharide antigens |
| Proposed epitopes | The epitope was proposed to be Terminal nonreducing portions of the O-chain |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other B. abortus strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 28-02-2010 |
| References | PMC261544 |
| PolysacDB ID | 1186 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-2 |
| Antibody type and class | IgG3 |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria |
| Proposed epitopes | This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other B. abortus strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 28-02-2010 |
| References | PMC261544 |
| PolysacDB ID | 1187 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-3 |
| Antibody type and class | IgG1 |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens |
| Proposed epitopes | The epitope was proposed to be Terminal nonreducing portions of the O-chain |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other B. abortus strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 28-02-2010 |
| References | PMC261544 |
| PolysacDB ID | 1188 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-4 |
| Antibody type and class | IgG3 |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria |
| Proposed epitopes | This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other B. abortus strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 01-03-2010 |
| References | PMC261544 |
| PolysacDB ID | 1189 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-5 |
| Antibody type and class | IgG1 |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab was specific to Y. enterocolitica and did not cross-react with Brucella abortus LPS |
| Proposed epitopes | The epitope was proposed to be partially directed to the core that gave rise to its specificity to Y. enterocolitica. The study also indicates that the portion of the O-antigen recognized is most probably an oligosaccharide portion which encompasses elements of both the O-chain and the core |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 01-03-2010 |
| References | PMC261544 |
| PolysacDB ID | 1190 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-6 |
| Antibody type and class | IgG3 |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab was specific to Y. enterocolitica and did not cross-react with Brucella abortus LPS |
| Proposed epitopes | The epitope was proposed to be partially directed to the core that gave rise to its specificity to Y. enterocolitica. The study also indicates that the portion of the O-antigen recognized is most probably an oligosaccharide portion which encompasses elements of both the O-chain and the core |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 02-03-2010 |
| References | PMC261544 |
| PolysacDB ID | 1191 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Yersinia enterocolitica 0:9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-antigen part consists of the following residues : -2)-α-D-Rhap4NFo-(1- |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Killed whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Ys.T9-7 |
| Antibody type and class | IgG2a |
| Assay System | Whole cell agglutination, immunodiffusion, immunoblotting and ELISA |
| Cross-reactivity | This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens |
| Proposed epitopes | The epitope was proposed to be Terminal nonreducing portions of the O-chain |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus. |
| Curator ID | AA + AS |
| Date of Curation | 02-03-2010 |
| References | PMC261544 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |