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PolysacDB ID | 1028 |
Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
BCSDB Structure | 130362 |
Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg] |
Antibodies | Mab BD1 |
Antibody Type Class | IgG1 |
Assay System | Indirect immunofluorescence |
Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and D. BD-1 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus |
Proposed Epitope | Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups |
IEDB Epitope | N/A |
Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
Curator ID | AA + AS |
Date of Curation | 08-01-2010 |
References | PMC259921 |
PolysacDB ID | 1029 |
Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
BCSDB Structure | 130362 |
Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg] |
Antibodies | Mab BA4 |
Antibody Type Class | IgM |
Assay System | Indirect immunofluorescence |
Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and B. BA4 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus |
Proposed Epitope | N\A |
IEDB Epitope | N/A |
Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
Curator ID | AA + AS |
Date of Curation | 08-01-2010 |
References | PMC259921 |
PolysacDB ID | 1030 |
Carbohydrate Name | O-deacetylated Glucurunoxylomannan (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Cryptococcus neoformans Serotype C (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
BCSDB Structure | 73 |
Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | GXM of C. neoformans serotype A 9759 was activated with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) and was coupled to BSA-AH. GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed (yield, 17 mg) |
Antibodies | Mab CD6 |
Antibody Type Class | IgG1 |
Assay System | Indirect immunofluorescence |
Cross Reactivity | This antibody cross-reacted with all 4 serotypes- A, B, C and D. |
Proposed Epitope | Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups |
IEDB Epitope | N/A |
Proposed Utility | The ability to produce a MAb against an epitope shared by many serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
Curator ID | AA + AS |
Date of Curation | 09-01-2010 |
References | PMC259921 |
PolysacDB ID | 1354 |
Carbohydrate Name | Tetrasaccharide of 3-deoxy-α-d-manno-oct-2-ulopyranosonic acid (Kdo) (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydophila psittaci (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | The allyl glycosides R-OCH2-CH=CH2 were conjugated with cysteamine yielding R-O(CH2)3-S-(CH2)2-NH3+, which were activated with thiophosgene into the isothiocyanate derivatives R O(CH2)3-S-(CH2)2-N=C=S and then conjugated to BSA yielding R-O(CH2)3-S-(CH2)2-NH-CS-NH-BSA, wherein R represents the glycosyl residue |
Antibodies | Mab S69-4 |
Antibody Type Class | IgG1 |
Assay System | IFT assay, ELISA |
Cross Reactivity | This antibody was specific only to C. psittaci |
Proposed Epitope | The epitope consists of a branched Kdo(2N/A8)[Kdo(2N/A4)] Kdo(2N/A4)Kdo(2N/A4) tetrasaccharide that is specific to Chlamydia psittaci |
IEDB Epitope | 76909 |
Proposed Utility | This Mab may provide insight into the molecular details of the interaction of these antibodies with acidic Kdo containing epitopes. Mab S69-4 can be used in immunohistology to reliably identify C. psittaci at the species level |
Curator ID | AA + AS |
Date of Curation | 26-06-2010 |
References | 16282606 |
PolysacDB ID | 1363 |
Carbohydrate Name | α-Kdo-(2->8)-α-Kdo-(2->4)-α-Kdo-(2->6)-β-GlcNAc-(l->6)-α-GlcNAc (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed Function | N/A |
Antigenic Nature | A-Kdo-(2->8)-a-Kdo-(2->4)-a-Kdo-(2->6)-P-GlcNAc-(l->6)-a-GlcNAc |
Carrier Name | Bovine serum albumin |
Conjugate Method | Derivatized using a solution of thiophosgene |
Antibodies | Mab S40-26 |
Antibody Type Class | N/A |
Assay System | ELISA, immunoblotting and immunofluorescence |
Cross Reactivity | N/A |
Proposed Epitope | N/A |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 28-06-2010 |
References | PMC258430 |
PolysacDB ID | 1364 |
Carbohydrate Name | α-Kdo-(2->8)-α-Kdo-(2->4)-α-Kdo-(2->6)-β-GlcNAc-(l->6)-α-GlcNAc (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed Function | N/A |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Derivatized using a solution of thiophosgene |
Antibodies | Mab S40-8 |
Antibody Type Class | N/A |
Assay System | ELISA, immunoblotting and immunofluorescence |
Cross Reactivity | N/A |
Proposed Epitope | N/A |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 29-06-2010 |
References | PMC258430 |
PolysacDB ID | 1366 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S23-24 |
Antibody Type Class | IgG1 |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis and salmonella minnesota R595 but did not cross-react with mycobacteria |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 29-06-2010 |
References | PMC256998 |
PolysacDB ID | 1367 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S25-7 |
Antibody Type Class | IgM |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis and salmonella minnesota R595 but did not cross-react with mycobacteria |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 29-06-2010 |
References | PMC256998 |
PolysacDB ID | 1368 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S25-27 |
Antibody Type Class | IgG1 |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595 |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 30-06-2010 |
References | PMC256998 |
PolysacDB ID | 1369 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S25-2 |
Antibody Type Class | IgG1 |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595 |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 30-06-2010 |
References | PMC256998 |
PolysacDB ID | 1370 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonioc acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S25-23 |
Antibody Type Class | IgG1 |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595 |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 30-06-2010 |
References | PMC256998 |
PolysacDB ID | 1371 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S25-5 |
Antibody Type Class | IgG1 |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595 |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 01-07-2010 |
References | PMC256998 |
PolysacDB ID | 1372 |
Carbohydrate Name | Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Chlamydia spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues |
BCSDB Structure | N/A |
Proposed Function | LPS represents one of the major surface antigens of this microorganism |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS) |
Antibodies | Mab S25-26 |
Antibody Type Class | IgG1 |
Assay System | Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting |
Cross Reactivity | This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595 |
Proposed Epitope | This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo |
IEDB Epitope | 76828 |
Proposed Utility | This Mab can be used in the epitope characterization of Chlamydial LPS |
Curator ID | AA + AS |
Date of Curation | 01-07-2010 |
References | PMC256998 |
PolysacDB ID | 1450 |
Carbohydrate Name | FVP2 polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Flammulina velutipes (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain |
BCSDB Structure | N/A |
Proposed Function | FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment |
Antibodies | Mab B11D |
Antibody Type Class | IgM |
Assay System | Indirect ELISA, Double Immunodiffusion |
Cross Reactivity | N/A |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens |
Curator ID | AA + AS |
Date of Curation | 14-07-2010 |
References | 19108616 |
PolysacDB ID | 1451 |
Carbohydrate Name | FVP2 polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Flammulina velutipes (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain |
BCSDB Structure | N/A |
Proposed Function | FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment |
Antibodies | Mab C8G |
Antibody Type Class | IgM |
Assay System | Indirect ELISA, Double Immunodiffusion |
Cross Reactivity | N/A |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens |
Curator ID | AA + AS |
Date of Curation | 14-07-2010 |
References | 19108616 |
PolysacDB ID | 1452 |
Carbohydrate Name | FVP2 polysaccharide (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Flammulina velutipes (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain |
BCSDB Structure | N/A |
Proposed Function | FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment |
Antibodies | Mab I6D |
Antibody Type Class | IgM |
Assay System | Indirect ELISA, Double Immunodiffusion |
Cross Reactivity | N/A |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens |
Curator ID | AA + AS |
Date of Curation | 21-07-2010 |
References | 19108616 |
PolysacDB ID | 1907 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab A7F |
Antibody Type Class | IgG3 |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 26-11-2010 |
References | 18991095 |
PolysacDB ID | 1908 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab L2D |
Antibody Type Class | IgM |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 26-11-2010 |
References | 18991095 |
PolysacDB ID | 1909 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab L6A |
Antibody Type Class | IgG3 |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 26-11-2010 |
References | 18991095 |
PolysacDB ID | 1910 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab M6C |
Antibody Type Class | IgM |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 26-11-2010 |
References | 18991095 |
PolysacDB ID | 1911 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab N3F |
Antibody Type Class | IgM |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 27-11-2010 |
References | 18991095 |
PolysacDB ID | 1912 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab P12B |
Antibody Type Class | IgG1 |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 27-11-2010 |
References | 18991095 |
PolysacDB ID | 1913 |
Carbohydrate Name | Marine fungal polysaccharide YCP (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain |
BCSDB Structure | N/A |
Proposed Function | YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor |
Antigenic Nature | Glycoconjugates |
Carrier Name | Bovine serum albumin |
Conjugate Method | 80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP |
Antibodies | Mab R10F |
Antibody Type Class | IgM |
Assay System | Inhibition ELISA, double immunodiffusion |
Cross Reactivity | This Mab was highly specific to P. herbarum YCP polysaccharide |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab is a promising drug candidate |
Curator ID | AA + AS |
Date of Curation | 27-11-2010 |
References | 18991095 |
PolysacDB ID | 2412 |
Carbohydrate Name | Phosphorylcholine (PC) (Drugpedia) |
Carbohydrate Class | Capsular polysaccharide |
Microbe Name | Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed Function | Antibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial |
Antigenic Nature | ASRNKANDYTTEYSASVKGRFIVS [peptide1] |
Carrier Name | Bovine serum albumin |
Conjugate Method | BSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C |
Antibodies | Antisera |
Antibody Type Class | N/A |
Assay System | ELISA, Opsonization and protection assays |
Cross Reactivity | N/A |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA |
Curator ID | AA + AS |
Date of Curation | 03-05-2011 |
References | 10992485 |
PolysacDB ID | 2461 |
Carbohydrate Name | Synthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8 (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe Name | Salmonella spp. (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Synthetic disaccharide abequose1-->3α-rhamnose |
BCSDB Structure | N/A |
Proposed Function | N/A |
Antigenic Nature | Glycoconjugate |
Carrier Name | Bovine serum albumin |
Conjugate Method | N/A |
Antibodies | Antisera |
Antibody Type Class | N/A |
Assay System | Immunofluorescence and co-agglutination (COA) |
Cross Reactivity | The antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria |
Proposed Epitope | N/A |
IEDB Epitope | N/A |
Proposed Utility | This antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci |
Curator ID | AA + AS |
Date of Curation | 23-05-2011 |
References | 373381 |
Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |