PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
   
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Record - 1 of 29   [TOP]

PolysacDB ID1028
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure130362
Proposed FunctionThe capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodGXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg]
AntibodiesMab BD1
Antibody Type ClassIgG1
Assay SystemIndirect immunofluorescence
Cross ReactivityThis antibody cross-reacted with 2 serotypes- A and D. BD-1 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus
Proposed EpitopeGlucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups
IEDB EpitopeN/A
Proposed UtilityMAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator IDAA + AS
Date of Curation08-01-2010
ReferencesPMC259921

Record - 2 of 29   [TOP]

PolysacDB ID1029
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure130362
Proposed FunctionThe capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodGXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg]
AntibodiesMab BA4
Antibody Type ClassIgM
Assay SystemIndirect immunofluorescence
Cross ReactivityThis antibody cross-reacted with 2 serotypes- A and B. BA4 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus
Proposed EpitopeN\A
IEDB EpitopeN/A
Proposed UtilityMAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator IDAA + AS
Date of Curation08-01-2010
ReferencesPMC259921

Record - 3 of 29   [TOP]

PolysacDB ID1030
Carbohydrate NameO-deacetylated Glucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype C   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure73
Proposed FunctionThe capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodGXM of C. neoformans serotype A 9759 was activated with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) and was coupled to BSA-AH. GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed (yield, 17 mg)
AntibodiesMab CD6
Antibody Type ClassIgG1
Assay SystemIndirect immunofluorescence
Cross ReactivityThis antibody cross-reacted with all 4 serotypes- A, B, C and D.
Proposed EpitopeGlucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups
IEDB EpitopeN/A
Proposed UtilityThe ability to produce a MAb against an epitope shared by many serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator IDAA + AS
Date of Curation09-01-2010
ReferencesPMC259921

Record - 4 of 29   [TOP]

PolysacDB ID1354
Carbohydrate NameTetrasaccharide of 3-deoxy-α-d-manno-oct-2-ulopyranosonic acid (Kdo)   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydophila psittaci   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodThe allyl glycosides R-OCH2-CH=CH2 were conjugated with cysteamine yielding R-O(CH2)3-S-(CH2)2-NH3+, which were activated with thiophosgene into the isothiocyanate derivatives R O(CH2)3-S-(CH2)2-N=C=S and then conjugated to BSA yielding R-O(CH2)3-S-(CH2)2-NH-CS-NH-BSA, wherein R represents the glycosyl residue
AntibodiesMab S69-4
Antibody Type ClassIgG1
Assay SystemIFT assay, ELISA
Cross ReactivityThis antibody was specific only to C. psittaci
Proposed EpitopeThe epitope consists of a branched Kdo(2N/A8)[Kdo(2N/A4)] Kdo(2N/A4)Kdo(2N/A4) tetrasaccharide that is specific to Chlamydia psittaci
IEDB Epitope76909
Proposed UtilityThis Mab may provide insight into the molecular details of the interaction of these antibodies with acidic Kdo containing epitopes. Mab S69-4 can be used in immunohistology to reliably identify C. psittaci at the species level
Curator IDAA + AS
Date of Curation26-06-2010
References16282606

Record - 5 of 29   [TOP]

PolysacDB ID1363
Carbohydrate Nameα-Kdo-(2->8)-α-Kdo-(2->4)-α-Kdo-(2->6)-β-GlcNAc-(l->6)-α-GlcNAc   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureA-Kdo-(2->8)-a-Kdo-(2->4)-a-Kdo-(2->6)-P-GlcNAc-(l->6)-a-GlcNAc
Carrier NameBovine serum albumin
Conjugate MethodDerivatized using a solution of thiophosgene
AntibodiesMab S40-26
Antibody Type ClassN/A
Assay SystemELISA, immunoblotting and immunofluorescence
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation28-06-2010
ReferencesPMC258430

Record - 6 of 29   [TOP]

PolysacDB ID1364
Carbohydrate Nameα-Kdo-(2->8)-α-Kdo-(2->4)-α-Kdo-(2->6)-β-GlcNAc-(l->6)-α-GlcNAc   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodDerivatized using a solution of thiophosgene
AntibodiesMab S40-8
Antibody Type ClassN/A
Assay SystemELISA, immunoblotting and immunofluorescence
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation29-06-2010
ReferencesPMC258430

Record - 7 of 29   [TOP]

PolysacDB ID1366
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S23-24
Antibody Type ClassIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis and salmonella minnesota R595 but did not cross-react with mycobacteria
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation29-06-2010
ReferencesPMC256998

Record - 8 of 29   [TOP]

PolysacDB ID1367
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-7
Antibody Type ClassIgM
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis and salmonella minnesota R595 but did not cross-react with mycobacteria
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation29-06-2010
ReferencesPMC256998

Record - 9 of 29   [TOP]

PolysacDB ID1368
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-27
Antibody Type ClassIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation30-06-2010
ReferencesPMC256998

Record - 10 of 29   [TOP]

PolysacDB ID1369
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-2
Antibody Type ClassIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation30-06-2010
ReferencesPMC256998

Record - 11 of 29   [TOP]

PolysacDB ID1370
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonioc acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-23
Antibody Type ClassIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation30-06-2010
ReferencesPMC256998

Record - 12 of 29   [TOP]

PolysacDB ID1371
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-5
Antibody Type ClassIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation01-07-2010
ReferencesPMC256998

Record - 13 of 29   [TOP]

PolysacDB ID1372
Carbohydrate NameTetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameChlamydia spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB StructureN/A
Proposed FunctionLPS represents one of the major surface antigens of this microorganism
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodAllylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
AntibodiesMab S25-26
Antibody Type ClassIgG1
Assay SystemHemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross ReactivityThis Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed EpitopeThis Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope76828
Proposed UtilityThis Mab can be used in the epitope characterization of Chlamydial LPS
Curator IDAA + AS
Date of Curation01-07-2010
ReferencesPMC256998

Record - 14 of 29   [TOP]

PolysacDB ID1450
Carbohydrate NameFVP2 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameFlammulina velutipes   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB StructureN/A
Proposed FunctionFVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodThe FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
AntibodiesMab B11D
Antibody Type ClassIgM
Assay SystemIndirect ELISA, Double Immunodiffusion
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator IDAA + AS
Date of Curation14-07-2010
References19108616

Record - 15 of 29   [TOP]

PolysacDB ID1451
Carbohydrate NameFVP2 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameFlammulina velutipes   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB StructureN/A
Proposed FunctionFVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodThe FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
AntibodiesMab C8G
Antibody Type ClassIgM
Assay SystemIndirect ELISA, Double Immunodiffusion
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator IDAA + AS
Date of Curation14-07-2010
References19108616

Record - 16 of 29   [TOP]

PolysacDB ID1452
Carbohydrate NameFVP2 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameFlammulina velutipes   (NCBI Taxonomy)   (Drugpedia)
Basic StructureConsists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB StructureN/A
Proposed FunctionFVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate MethodThe FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
AntibodiesMab I6D
Antibody Type ClassIgM
Assay SystemIndirect ELISA, Double Immunodiffusion
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator IDAA + AS
Date of Curation21-07-2010
References19108616

Record - 17 of 29   [TOP]

PolysacDB ID1907
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab A7F
Antibody Type ClassIgG3
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation26-11-2010
References18991095

Record - 18 of 29   [TOP]

PolysacDB ID1908
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab L2D
Antibody Type ClassIgM
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation26-11-2010
References18991095

Record - 19 of 29   [TOP]

PolysacDB ID1909
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab L6A
Antibody Type ClassIgG3
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation26-11-2010
References18991095

Record - 20 of 29   [TOP]

PolysacDB ID1910
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab M6C
Antibody Type ClassIgM
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation26-11-2010
References18991095

Record - 21 of 29   [TOP]

PolysacDB ID1911
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab N3F
Antibody Type ClassIgM
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation27-11-2010
References18991095

Record - 22 of 29   [TOP]

PolysacDB ID1912
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab P12B
Antibody Type ClassIgG1
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation27-11-2010
References18991095

Record - 23 of 29   [TOP]

PolysacDB ID1913
Carbohydrate NameMarine fungal polysaccharide YCP   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NamePhoma herbarum YS4108   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB StructureN/A
Proposed FunctionYCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic NatureGlycoconjugates
Carrier NameBovine serum albumin
Conjugate Method80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
AntibodiesMab R10F
Antibody Type ClassIgM
Assay SystemInhibition ELISA, double immunodiffusion
Cross ReactivityThis Mab was highly specific to P. herbarum YCP polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab is a promising drug candidate
Curator IDAA + AS
Date of Curation27-11-2010
References18991095

Record - 24 of 29   [TOP]

PolysacDB ID2412
Carbohydrate NamePhosphorylcholine (PC)   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameStreptococcus pneumoniae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed FunctionAntibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial
Antigenic NatureASRNKANDYTTEYSASVKGRFIVS [peptide1]
Carrier NameBovine serum albumin
Conjugate MethodBSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C
AntibodiesAntisera
Antibody Type ClassN/A
Assay SystemELISA, Opsonization and protection assays
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA
Curator IDAA + AS
Date of Curation03-05-2011
References10992485

Record - 25 of 29   [TOP]

PolysacDB ID2461
Carbohydrate NameSynthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameSalmonella spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureSynthetic disaccharide abequose1-->3α-rhamnose
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugate
Carrier NameBovine serum albumin
Conjugate MethodN/A
AntibodiesAntisera
Antibody Type ClassN/A
Assay SystemImmunofluorescence and co-agglutination (COA)
Cross ReactivityThe antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci
Curator IDAA + AS
Date of Curation23-05-2011
References373381

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036