PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1623
Carbohydrate NameLipooligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameMoraxella catarrhalis Serotype A strain 25238   (NCBI Taxonomy)   (Drugpedia)
Basic StructureLOS consists of an oligosaccharide and lipid A and is similar to the lipopolysaccharide (LPS) of gramnegative enteric pathogens, but it lacks the O-antigenic side chain of repeating units characteristic of classical LPS. The oligosaccharide part consists of the following residues : α-D-Galp-(1-->4)-β-D-Galp-(1-->4)-α-D-Glcp-(1-->2)-β-D-Glcp-(1-->6)-α-D-Glcp [branched to α-D-GlcpNAc-(1-->2)-β-D-Glcp-(1-->4)] and [branched to β-D-Glcp-(1-->3)] -(1-->5)-Kdo
BCSDB StructureN/A
Proposed FunctionMoracella lipooligosaccharide is a potential virulence factor
Antigenic NatureGlycoconjugates
Carrier NameCross-reactive mutant (CRM9) of diphtheria toxin
Conjugate MethodAdipic acid dihydrazide (ADH) was bound to detoxified lipooligosaccharide [LOS] to form adipic hydrazide (AH)-dLOS derivatives, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). dLOS (70 mg) was dissolved in 7 ml of 345 mM ADH (molar ratio of ADH to LOS is 100 to 1, based on an estimated Mr of 3,000 for dLOS). Sulfo-NHS was added to a concentration of 8 mM, the pH was adjusted to 4.8, and EDC was added to a concentration of 0.1 M. The reaction mixture was stirred and maintained at pH 4.8 for 3 h. The reaction mixture was adjusted to pH 7.0 and passed through the G-50 column as described above. The eluate was assayed for carbohydrate and for AH. The peaks containing both carbohydrate and AH were pooled, freeze-dried, and designated AH-dLOS. AH-dLOS was measured for its composition, using dLOS and ADH as standards
AntibodiesPolysera
Antibody Type ClassN/A
Assay SystemEnzyme-linked immunospot assay, ELISA and Bacterial challenge assay
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThe dLOS-CRM vaccine induced a significant bacterial clearance (70 to 90%) of both homologous and heterologous strains in the lungs compared to that observed in the controls
Curator IDAA + AS
Date of Curation25-09-2010
ReferencesPMC130355

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036