PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Page - 1 of 1

Record - 1 of 4   [TOP]

PolysacDB ID1571
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameLeptospira interrogans serovar pomona   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe oligosaccharide contained rhamnose, ribose, glucose, and glucosamine
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxoid
Conjugate MethodThe oligosaccharide was modified as follows : it was dissolved in N-ethylmorpholine-water-12 N HCl (2.5:6.5:1 [vol/vol/vol], pH 8.5) in concentrations of approximately 24 umol in 250ul. 125umol of N-succinimidyl S-acetylmercaptoacetate in 250ul of N,N dimethylacetamide was added to the oligosaccharide. This solution was added to modified diphtheria toxoid
AntibodiesPolysera
Antibody Type ClassIgG and IgM
Assay SystemEnzyme Immunoassay, immunoblotting and dot blot
Cross ReactivityThis antisera cross-reacted with LPS from some other leptospiral serovars (serovars hardjobovis, ballum, copenhageni, canicola, grippotyphosa, and batavia) and LPS from other bacteria (Salmonella typhimurium and the Re mutant of Shigella flexneri) and did not react with serovars australis, grippotyphosa, ballum, or tarassovi
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis antiserum induced a high CL response in mouse macrophage monolayers
Curator IDAA + AS
Date of Curation10-09-2010
ReferencesPMC303291

Record - 2 of 4   [TOP]

PolysacDB ID2639
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype D [strain 24067]   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB StructureN/A
Proposed FunctionAntiphagocytic ; an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxoid
Conjugate Method5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
AntibodiesMab G14
Antibody Type ClassIgM
Assay SystemELISA and immunofluorescence
Cross ReactivityThis antibody cross-reacted with Serotypes A and D
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis MAb mediated complement deposition on solid phase GXM by ELISA
Curator IDAA + AS
Date of Curation28-07-2011
References15271943

Record - 3 of 4   [TOP]

PolysacDB ID2640
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype D [strain 24067]   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB StructureN/A
Proposed FunctionAntiphagocytic ; an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxoid
Conjugate Method5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
AntibodiesMab G15
Antibody Type ClassIgM
Assay SystemELISA and immunofluorescence
Cross ReactivityThis antibody cross-reacted with Serotypes A and D in whole cell ELISA and immunofluorescence. Did not cross-react with Serotype A glucurunoxylomannan alone
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis MAb mediated complement deposition on solid phase GXM by ELISA. Administration of Mab G15 also prolonged the survival of C. neoformans
Curator IDAA + AS
Date of Curation29-07-2011
References15271943

Record - 4 of 4   [TOP]

PolysacDB ID2641
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype D [strain 24067]   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB StructureN/A
Proposed FunctionAntiphagocytic ; an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxoid
Conjugate Method5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid
AntibodiesMab G19
Antibody Type ClassIgM
Assay SystemELISA and immunofluorescence
Cross ReactivityThis antibody cross-reacted with Serotypes A and D
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis MAb mediated complement deposition on solid phase GXM by ELISA
Curator IDAA + AS
Date of Curation29-07-2011
References15271943

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036