PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1014
Carbohydrate NameGlucan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureGlucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
BCSDB Structure125281
Proposed FunctionGlucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxoid CRM 197
Conjugate MethodA 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices
AntibodiesMab 2G8
Antibody Type ClassIgG2
Assay SystemFlow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay
Cross ReactivityThis antibody binds both C. neoformans and Candida albicans
Proposed EpitopeN\A
IEDB Epitope76671
Proposed UtilityMAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo.
Curator IDAA + AS
Date of Curation04-01-2010
ReferencesPMC2168274

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PolysacDB ID1015
Carbohydrate NameBeta-glucan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureGlucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
BCSDB Structure125281
Proposed FunctionGlucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxoid CRM 197
Conjugate MethodA 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices
AntibodiesPolysera
Antibody Type ClassIgG
Assay SystemInvivo protection assays, growth inhibition assays, indirect ELISA
Cross ReactivityN/A
Proposed EpitopeN\A
IEDB Epitope76671
Proposed UtilityThe Lam-CRM conjugate is immunogenic and protective against systemic candidiasis in mice. The antibodies induce passive protection in mice against systemic and mucosal candidiasis
Curator IDAA + AS
Date of Curation04-01-2010
ReferencesPMC2212864

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