PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID2642
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype D   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThis polysaccharide is a high m.w. polymer with a linear α-(1-->3)-linked mannose backbone that is substituted with non-reducing D-xyIosyal and D-glucosyluronic acid groups. O-Acetylation varies with the serotype. Type D is the most heavily O-acetylated
BCSDB Structure136943
Proposed FunctionAntiphagocytic ; an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameMethylated bovine serum albumin
Conjugate MethodA saline solution containing 500 pg of cryptococcal polysaccharide was mixed with 500 pg aqueous methylated bovine serum albumin. The volume was brought to 2 ml with sterile distilled water
AntibodiesPolysera
Antibody Type ClassIgG
Assay SystemAntiserum to untreated cryptococcal polysaccharide was enriched for O-acetyl and carboxyl specific antibody respectively. The antisera was then used in ouchterlony double diffusion.
Cross ReactivityN/A
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityO-acetyl and carboxyl groups are strong and distinct physicochemical determinants on cryptococcal polysaccharide. The enriched antiserum displayed a level of opsonic activity that was similar to non-enriched antiserum. This showed that neither O-acetyl nor carboxyl groups were responsible for inhibition of phagocytosis by cryptococcal polysaccharide
Curator IDAA + AS
Date of Curation30-07-2011
References15271943

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036