PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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PolysacDB ID	1013
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM backbone consists of a linear α(1-->3)-linked mannan substituted at 2-O positions by single residues of either xylose or glucuronic acid
BCSDB Structure	116826
Proposed Function	Antiphagocytic ; an important virulence factor
Antigenic Nature	Pure Capsular polysaccharide
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab E1
Antibody Type Class	IgG1
Assay System	Agglutination with C. neoformans serotype A cells, Radial immunodiffusion, Indirect Immunofluorescence,, ELISA, Competitive binding assays
Cross Reactivity	This antibody was highly specific to serotype A, low levels of cross reactivity to serotypes B and D. Among the other yeasts tested, a cross-reaction was only detected with Trichosporon beigelii.
Proposed Epitope	N\A
IEDB Epitope	115576
Proposed Utility	Could be useful for fundamental studies on the glucuronoxylomannan structure, as well as for clinical applications such as serotyping and possibly the serological diagnosis of cryptococcosis
Curator ID	AA + AS
Date of Curation	04-01-2010
References	PMC260404

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PolysacDB ID	1016
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 21D2
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotype A, D and GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1017
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 14A12
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1018
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 11E2
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1019
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 7B13
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1020
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 12G5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

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PolysacDB ID	1021
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 20C5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

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PolysacDB ID	1022
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 20B5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

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PolysacDB ID	1023
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 4H3
Antibody Type Class	IgG3
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC2118886

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PolysacDB ID	1024
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 15C6
Antibody Type Class	IgA
Assay System	Immunofluorescence
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC2118886

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PolysacDB ID	1037
Carbohydrate Name	Galactomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units
BCSDB Structure	N/A
Proposed Function	Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs
Antigenic Nature	Formalinized spores of A. fumigatus.
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab EB-A2
Antibody Type Class	IgM
Assay System	Indirect and Competitive ELISA
Cross Reactivity	This antibody cross-reacted to P. digitatum and Penicillium marneffei speices.
Proposed Epitope	3(1-->5)galactofuranose residues of galactomannan
IEDB Epitope	N/A
Proposed Utility	EB-A2 has been used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition. The sensitivity (95%), specificity (100%), positive predictive value (100%), and negative predictive value (99%)of the ELISA
Curator ID	AA + AS
Date of Curation	10-01-2010
References	PMC257149

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PolysacDB ID	1039
Carbohydrate Name	Capsular polysaccharides K5 (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Escherichia coli (NCBI Taxonomy) (Drugpedia)
Basic Structure	Capsular polysacchirides K5 is disaccharide with repeating unit 4-β-GlcUA(1-->4)-α-GlcNAc
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharides are responsible for pathogen`s virulence
Antigenic Nature	Whole formalinized E.coli cells.
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 865
Antibody Type Class	IgM
Assay System	Indirect ELISA, Immunoblot analysis
Cross Reactivity	This antibody was cross-reactive with ECA of E.coli and S.typhimurium strains.
Proposed Epitope	4-β-GlcUA(1-->4)-α-GlcNAc
IEDB Epitope	N/A
Proposed Utility	Reaction pattern of Mab with whole cell bacteria indicate that ECA and outer core in encapsulated bacteria are not readily accessible to antibodies.This suggests that these common envelop structures may not be useful antigens for vaccine development.
Curator ID	AA + AS
Date of Curation	11-01-2010
References	PMC261975

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PolysacDB ID	1043
Carbohydrate Name	Galactomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units
BCSDB Structure	N/A
Proposed Function	Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs
Antigenic Nature	Cold alkali extract of mycelium
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 40
Antibody Type Class	IgM
Assay System	Indirect Enzyme Immunoassay, immunofluorescence, Preciptin assay
Cross Reactivity	This antibody was cross-reactive with C.albicans
Proposed Epitope	Mannopyranosyl side chain
IEDB Epitope	N/A
Proposed Utility	This antibody can be used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition.
Curator ID	AA + AS
Date of Curation	13-01-2010
References	PMC258783

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PolysacDB ID	1044
Carbohydrate Name	Galactomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units
BCSDB Structure	N/A
Proposed Function	Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs
Antigenic Nature	Cold alkali extract of mycelium
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 1
Antibody Type Class	IgM
Assay System	Indirect Enzyme Immunoassay, immunofluorescence, Preciptin assay
Cross Reactivity	This antibody was cross-reactive with C.albicans
Proposed Epitope	Oligogalactoside side chain
IEDB Epitope	N/A
Proposed Utility	This antibody can be used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition.
Curator ID	AA + AS
Date of Curation	13-01-2010
References	PMC258783

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PolysacDB ID	1171
Carbohydrate Name	Lipoologosaccharide band A of the LPS (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Bordetella pertusis (NCBI Taxonomy) (Drugpedia)
Basic Structure	B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B
BCSDB Structure	N/A
Proposed Function	The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab G10F8C3
Antibody Type Class	IgG3
Assay System	Immunoblotting, Protein A sepharose affinity chromatography, bactericidal assays
Cross Reactivity	This antibody was specific to B. pertusis strains
Proposed Epitope	This Mab reacts with the core oligosaccharide moeity
IEDB Epitope	N/A
Proposed Utility	This Mab exhibits complement dependent bactericidal activity against strains of B. pertussis that express the LOS A phenotype. In addition it was effective in reducing colonization by B. pertussis in both the lungs and tracheas of mice after aerosol infection
Curator ID	AA + AS
Date of Curation	25-02-2010
References	PMC258314

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PolysacDB ID	1472
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 73-30
Antibody Type Class	IgM
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains DU1, DU2, GU2 and CA6, GU9 and CA4 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	28-07-2010
References	PMC170591

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PolysacDB ID	1473
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab BG-7 (P12)
Antibody Type Class	IgM
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains DU1, DU2, GU2 and CA6, GU9 and CA4 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	28-07-2010
References	PMC170591

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PolysacDB ID	1474
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab H18A
Antibody Type Class	IgG3
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains CG4 and CA2, CG9 and GU10 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	28-07-2010
References	PMC170591

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PolysacDB ID	1475
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab BG-8 (F3)
Antibody Type Class	IgM
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains CG4 and CA2, CG9 and GU10 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Record - 20 of 36 [TOP]

PolysacDB ID	1476
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	MAB 2108 (7LE)
Antibody Type Class	IgG1
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains CG9 and GU10, GU2 and CA6 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Record - 21 of 36 [TOP]

PolysacDB ID	1477
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab BG-5 (T174)
Antibody Type Class	IgG1
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains CG9 and GU10, GU2 and CA6 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Record - 22 of 36 [TOP]

PolysacDB ID	1478
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed Function	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab BG-6 (T218)
Antibody Type Class	IgM
Assay System	Immunoblotting and ELISA
Cross Reactivity	This anti-Lex Mab reacted with strains CG4 and CA2, CG9 and GU10 and did not react with strains 43504-r and CG7
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Record - 23 of 36 [TOP]

PolysacDB ID	1479
Carbohydrate Name	Le^x carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure consists of the following residues : (Galβ1-->4[Fucα1-->3]GlcNAc
BCSDB Structure	10702
Proposed Function	N/A
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 85-5000
Antibody Type Class	IgM
Assay System	Immunoblotting, immunoelectron microscopy and ELISA
Cross Reactivity	This Lex specific Mab cross-reacted with H. pylori strains NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206
Proposed Epitope	Galβ1-->4[Fucα1-->3]GlcNAc
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	30-07-2010
References	PMC173655

Record - 24 of 36 [TOP]

PolysacDB ID	1480
Carbohydrate Name	Le^x carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab CNM 1336
Antibody Type Class	IgM
Assay System	Immunoblotting, immunoelectron microscopy and ELISA
Cross Reactivity	This Lex specific Mab cross-reacted with H. pylori strains NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206
Proposed Epitope	Galβ1-->4[Fucα1-->3]GlcNAcβ1-->3Galβ1-->4Glc
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	30-07-2010
References	PMC173655

Record - 25 of 36 [TOP]

PolysacDB ID	1481
Carbohydrate Name	Galβ1-->4[Fucα1-->3]GlcNAcβ1--> 3Galβ1-->4[Fucα1-->3]GlcNAc (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure consists of the following residues : Galβ1-->4[Fucα1-->3]GlcNAcβ1--> 3Galβ1-->4[Fucα1-->3]GlcNAc
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	N/A
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab BCR 90/45
Antibody Type Class	IgM
Assay System	Immunoblotting, immunoelectron microscopy and ELISA
Cross Reactivity	This Lex specific Mab cross-reacted with H. pylori strains NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206
Proposed Epitope	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	30-07-2010
References	PMC173655

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036