PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1025
Carbohydrate NameCapsular polysaccharide A   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB StructureN/A
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 439
Antibody Type ClassIgG1
Assay SystemOuchterlony double immunodiffusion, Phagocytosis assays
Cross ReactivityThis antibody cross-reacted with all the 4 serotypes- A, B, C and D
Proposed EpitopeThe reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide
IEDB EpitopeN/A
Proposed UtilityMAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator IDAA + AS
Date of Curation07-01-2010
ReferencesPMC260621

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PolysacDB ID1026
Carbohydrate NameCapsular polysaccharide A   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB StructureN/A
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 1255
Antibody Type ClassIgG1
Assay SystemOuchterlony double immunodiffusion, Phagocytosis assays
Cross ReactivityThis antibody cross-reacted with 3 serotypes- A, B and D
Proposed EpitopeThe reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide
IEDB EpitopeN/A
Proposed UtilityMAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator IDAA + AS
Date of Curation08-01-2010
ReferencesPMC260621

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PolysacDB ID1027
Carbohydrate NameCapsular polysaccharide D   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB StructureN/A
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 302
Antibody Type ClassIgG1
Assay SystemOuchterlony double immunodiffusion, Phagocytosis assays
Cross ReactivityThis antibody cross-reacted with 2 serotypes- A and D
Proposed EpitopeThe reactivity of this antibody required acetylation of the parent polysaccharide. Hence proposed epitopes may be O-acetyl groups
IEDB EpitopeN/A
Proposed UtilityMAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator IDAA + AS
Date of Curation08-01-2010
ReferencesPMC260621

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PolysacDB ID2601
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure130477
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 339
Antibody Type ClassN/A
Assay SystemELISA and Immunodiffusion
Cross ReactivityThis antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes
Curator IDAA + AS
Date of Curation15-07-2011
References2476401

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PolysacDB ID2602
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype D   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure136943
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 386
Antibody Type ClassN/A
Assay SystemELISA and Immunodiffusion
Cross ReactivityThis antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA
Proposed EpitopeSince Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes
Curator IDAA + AS
Date of Curation15-07-2011
References2476401

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PolysacDB ID2603
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure130477
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 471
Antibody Type ClassN/A
Assay SystemELISA and Immunodiffusion
Cross ReactivityThis antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes. This monoclonal antibody promoted opsonization of Cryptococcus cells
Curator IDAA + AS
Date of Curation16-07-2011
References2476401

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PolysacDB ID2604
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype C   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure136942
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 3C2
Antibody Type ClassN/A
Assay SystemELISA and Immunodiffusion
Cross ReactivityThis antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes. This monoclonal antibody promoted opsonization of Cryptococcus cells
Curator IDAA + AS
Date of Curation16-07-2011
References2476401

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PolysacDB ID2612
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure116826
Proposed FunctionThe capsular polysaccharide is an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 1326
Antibody Type ClassIgG1
Assay SystemELISA
Cross ReactivityThis antibody was cross-reactive to serotypes A and D
Proposed EpitopeN/A
IEDB Epitope115576
Proposed UtilityThis monoclonal antibody promoted opsonization of Cryptococcus cells
Curator IDAA + AS
Date of Curation18-07-2011
References1583327

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PolysacDB ID2637
Carbohydrate NameGlucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans Serotype B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure9354
Proposed FunctionAntiphagocytic ; an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab F10F5
Antibody Type ClassIgG1
Assay SystemELISA
Cross ReactivityThis antibody cross-reacted with Serotypes A and B
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan
Curator IDAA + AS
Date of Curation28-07-2011
References12965925

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PolysacDB ID2638
Carbohydrate NameDe-O-acetylated Glucurunoxylomannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCryptococcus neoformans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
BCSDB Structure9353
Proposed FunctionAntiphagocytic ; an important virulence factor
Antigenic NatureGlycoconjugates
Carrier NameSheep erythrocytes
Conjugate MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab F12D2
Antibody Type ClassIgG3
Assay SystemELISA
Cross ReactivityThis antibody cross-reacted with Serotypes A, B, C and D
Proposed EpitopeStudies on this antibody showed that there are 2 types of epitopes on GXM-O-acetyl dependent and O-acetyl independent. MAb F12D2 also fails to react with xylose deficient GXM shopwing that xylose residues form an important part of the epitope
IEDB EpitopeN/A
Proposed UtilityThis antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan. This antibody showed that the induction of a MAb that is reactive with all serotypes does not inherently require the presence of an O-acetyl group on the immunizing GXM
Curator IDAA + AS
Date of Curation28-07-2011
References12965925

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036