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| PolysacDB ID | 1001 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 116826 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates. |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA), tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Polysera |
| Antibody Type Class | IgM |
| Assay System | Double immunodiffusion, ELISA |
| Cross Reactivity | Polsera cross-reacted with capsular polysaccharides of C neoformans serotype A and D |
| Proposed Epitope | O-acetyl groups, glucuronyl residues |
| IEDB Epitope | 115576 |
| Proposed Utility | The conjugate vaccines prepared through hydroxyl activation of glucurunoxylomannan are sufficiently immunogenic and appear to be suitable for clinical evaluation |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | 1716613 |
| PolysacDB ID | 1002 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates. |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 2E9 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A, B and D |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | PMC173409 |
| PolysacDB ID | 1003 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | N/A |
| Proposed Function | Activates leukocytes, stimulating their phagocytic, cytotoxic, and antimicrobial activities, including the production of reactive oxygen and nitrogen intermediates |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | The polysaccharide was activated with CDAP. A 60-ml volume of CDAP (100 mg/ml of acetonitrile) was added to a solution of polysaccharide (2 ml, 10 mg of polysaccharide per ml of PFS) at room temperature. The pH was maintained at 5.8 to 6.0 for 30 s, and 60 ml of 0.2 M TEA was added to a pH of 7.0. The reaction was carried out for 2 min, and an equal volume of 0.8 M adipic acid dihydrazide [ADH] in 0.5 M NaHCO3 was added. This reaction was carried out for 2 h, and the pH was maintained at 8.0 to 8.5 with 0.1 N NaOH. The reaction mixture was dialyzed against PFS and passed through a column (3 by 46 cm) of P-10 in PFW. The void volume fractions were pooled, freeze-dried. ADH-derivatized polysaccharide (10 mg) was dissolved in PFS (2 ml). An equal weight of protein was added, and the pH was maintained at 5.1 to 5.5 with 0.1 M HCl. The reaction mixture was put on ice, EDAC was added to a final concentration of 0.05 M, and the pH was maintained at 5.1 to 5.5 for 4 h in 0.1 M HCl. The reaction mixtures were dialyzed against 0.2 M NaCl for 2 days with three changes of outer fluid and were passed through a column (1.5 by 90 cm) of Sepharose CL-6B in 0.2 M NaCl. The void volume fractions were stored at 3 to 88°C |
| Antibodies | Mab 24c5 |
| Antibody Type Class | IgG |
| Assay System | ELISA, Immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with M. kansasii, BCG Pasteur, M. smegmatis, M. phlei, M. fortuitum, and M. avium |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | The antibody was used to study M. tuberculosis Glucan expression in vitro and in vivo |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | PMC127896 |
| PolysacDB ID | 1004 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Haemophilus influenzae type B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate |
| BCSDB Structure | 23423 |
| Proposed Function | An important virulence determinant |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Neisseria meningitidis group B outer membrane protein complex (Hib PS-OMP) |
| Conjugate Method | The strategy uses a oligopeptide spacer molecule whose parts are derived from both modified Haemophilus influenzae capsular polysaccharide and Neisseria meningitides group B outer membrane complex |
| Antibodies | Polysera |
| Antibody Type Class | IgG1 and IgG2 |
| Assay System | N\A |
| Cross Reactivity | N/A |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | These antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | PMC296547 |
| PolysacDB ID | 1005 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Haemophilus influenzae type B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate |
| BCSDB Structure | 23423 |
| Proposed Function | An important virulence determinant |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | Protein solutions (25 mg/ml) and Adipic acid dihydrazide [ADH] (3.45 rag/rag protein) were reacted with three different concentrations of EDAC (0 1, 0.3, and 0.6 rag/rag protein) The pH of the reaction mixture was maintained at 4.7 ± 0.2 with 0.1 N HC! The reaction proceeded at room temperature for 3 h and the reaction mixtures were dialyzed at 3-8°C with two changes/d against 6 liter of 0 2 M NaCI. The albumin and polysaccharide derivatives were then dialyzed against two 6-liter changes of deionized water and freeze-dried. The polysaccharide was activated with CNBr. Briefly, a solution of polysaccharide (5.0 mg/ml), equilibrated at 4°C, was rapidly brought to pH 10.5 with 0.1 N NaOH. 100 mg/ml CNBr was added to a final concentration of 0.4 mg/mg polysaccharide, and the pH maintained at 10.5 for 6 rain. Then the reaction mixture was brought to pH 8 5 with 0.5 M NaHCO3, and the CNBr-actlvated polysaccharide added to an equal weight of ADH-protein. The reaction mixture was tumbled gently overnight at 3-8°C and then centrifuged at 16,000 g, 4°C for 20 ram. The supernatant was passed through a CL-4B Sepharose column, 1 5 × 90 cm, that was equihbrated with 0 2 M ammonium acetate. The void-volume fractions were pooled, dialyzed against 0.01 M phosphate-buffered 0.145 M NaCI, pH 7.0, at 3-8°C, and passed through a 045-nm membrane and stored at 3-8°C |
| Antibodies | Polysera |
| Antibody Type Class | IgG and IgA |
| Assay System | Radioimmunoassay |
| Cross Reactivity | N/A |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | These antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis |
| Curator ID | AA + AS |
| Date of Curation | 02-01-2010 |
| References | PMC2185954 |
| PolysacDB ID | 1006 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 3B6 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotypes A, B, C and D |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections |
| Curator ID | AA + AS |
| Date of Curation | 02-01-2010 |
| References | PMC173409 |
| PolysacDB ID | 1012 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Streptococcus Group B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1- |
| BCSDB Structure | 6237 |
| Proposed Function | Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8°C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Polysera |
| Antibody Type Class | Mainly IgG, particularly IgG1 and IgG3 |
| Assay System | Double immunodiffusion, capillary precipitation, ELISA |
| Cross Reactivity | N/A |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC258520 |
| PolysacDB ID | 1013 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM backbone consists of a linear α(1-->3)-linked mannan substituted at 2-O positions by single residues of either xylose or glucuronic acid |
| BCSDB Structure | 116826 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Pure Capsular polysaccharide |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab E1 |
| Antibody Type Class | IgG1 |
| Assay System | Agglutination with C. neoformans serotype A cells, Radial immunodiffusion, Indirect Immunofluorescence,, ELISA, Competitive binding assays |
| Cross Reactivity | This antibody was highly specific to serotype A, low levels of cross reactivity to serotypes B and D. Among the other yeasts tested, a cross-reaction was only detected with Trichosporon beigelii. |
| Proposed Epitope | N\A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could be useful for fundamental studies on the glucuronoxylomannan structure, as well as for clinical applications such as serotyping and possibly the serological diagnosis of cryptococcosis |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC260404 |
| PolysacDB ID | 1014 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Candida albicans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | 125281 |
| Proposed Function | Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid CRM 197 |
| Conjugate Method | A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices |
| Antibodies | Mab 2G8 |
| Antibody Type Class | IgG2 |
| Assay System | Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay |
| Cross Reactivity | This antibody binds both C. neoformans and Candida albicans |
| Proposed Epitope | N\A |
| IEDB Epitope | 76671 |
| Proposed Utility | MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo. |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC2168274 |
| PolysacDB ID | 1015 |
| Carbohydrate Name | Beta-glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Candida albicans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | 125281 |
| Proposed Function | Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid CRM 197 |
| Conjugate Method | A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices |
| Antibodies | Polysera |
| Antibody Type Class | IgG |
| Assay System | Invivo protection assays, growth inhibition assays, indirect ELISA |
| Cross Reactivity | N/A |
| Proposed Epitope | N\A |
| IEDB Epitope | 76671 |
| Proposed Utility | The Lam-CRM conjugate is immunogenic and protective against systemic candidiasis in mice. The antibodies induce passive protection in mice against systemic and mucosal candidiasis |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC2212864 |
| PolysacDB ID | 1016 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 21D2 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A, D and GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 05-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1017 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 14A12 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 05-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1018 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 11E2 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 05-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1019 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 7B13 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 05-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1020 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 12G5 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 06-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1021 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 20C5 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 06-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1022 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 20B5 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 06-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1023 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 4H3 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 07-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1024 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 113471 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Cryptococci cells |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 15C6 |
| Antibody Type Class | IgA |
| Assay System | Immunofluorescence |
| Cross Reactivity | This antibody was specific to D serotype and cross-reacted with GH strain |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was shown to bind to cryptococcal capsule |
| Curator ID | AA + AS |
| Date of Curation | 07-01-2010 |
| References | PMC2118886 |
| PolysacDB ID | 1025 |
| Carbohydrate Name | Capsular polysaccharide A (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | N/A |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 439 |
| Antibody Type Class | IgG1 |
| Assay System | Ouchterlony double immunodiffusion, Phagocytosis assays |
| Cross Reactivity | This antibody cross-reacted with all the 4 serotypes- A, B, C and D |
| Proposed Epitope | The reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 07-01-2010 |
| References | PMC260621 |
| PolysacDB ID | 1026 |
| Carbohydrate Name | Capsular polysaccharide A (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | N/A |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 1255 |
| Antibody Type Class | IgG1 |
| Assay System | Ouchterlony double immunodiffusion, Phagocytosis assays |
| Cross Reactivity | This antibody cross-reacted with 3 serotypes- A, B and D |
| Proposed Epitope | The reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC260621 |
| PolysacDB ID | 1027 |
| Carbohydrate Name | Capsular polysaccharide D (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | N/A |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 302 |
| Antibody Type Class | IgG1 |
| Assay System | Ouchterlony double immunodiffusion, Phagocytosis assays |
| Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and D |
| Proposed Epitope | The reactivity of this antibody required acetylation of the parent polysaccharide. Hence proposed epitopes may be O-acetyl groups |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC260621 |
| PolysacDB ID | 1028 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
| BCSDB Structure | 130362 |
| Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugate Method | GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg] |
| Antibodies | Mab BD1 |
| Antibody Type Class | IgG1 |
| Assay System | Indirect immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and D. BD-1 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus |
| Proposed Epitope | Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC259921 |
| PolysacDB ID | 1029 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
| BCSDB Structure | 130362 |
| Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugate Method | GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg] |
| Antibodies | Mab BA4 |
| Antibody Type Class | IgM |
| Assay System | Indirect immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and B. BA4 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC259921 |
| PolysacDB ID | 1030 |
| Carbohydrate Name | O-deacetylated Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype C (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
| BCSDB Structure | 73 |
| Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugate Method | GXM of C. neoformans serotype A 9759 was activated with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) and was coupled to BSA-AH. GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed (yield, 17 mg) |
| Antibodies | Mab CD6 |
| Antibody Type Class | IgG1 |
| Assay System | Indirect immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with all 4 serotypes- A, B, C and D. |
| Proposed Epitope | Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups |
| IEDB Epitope | N/A |
| Proposed Utility | The ability to produce a MAb against an epitope shared by many serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 09-01-2010 |
| References | PMC259921 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |