PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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PolysacDB ID	1232
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Brucella abortus S19 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Core oligosaccharide is composed of mannose, glucose, 2-amino-2,6-dideoxyglucose (quinovosamine), 2-amino-2-deoxyglucose (GlcN),3-deoxy-D-manno-2-octulosonic acid (KDO), and unidentified sugars. The core is linked to the lipid A, which in contrast to most LPS studied, contains both GlcN and 2,3- diamino-2,3-dideoxyglucose (GlcN3N) as backbone sugars and amide- and ester-linked long-chain saturated (C16:0 to C18:0) and hydroxylated (3-OH-C12:0, to 29-OH-C30:0) fatty acids
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Acetone-killed B. abortus S19 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Baro-1
Antibody Type Class	IgG3
Assay System	Enzyme Immunoassay,immunoblotting
Cross Reactivity	Baro1 Mab showed cross-reactivity against B. melitiensis, B. canis and B. ovis LPS. Did not cross-react wirth S. coli and S. minnesota
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator ID	AA + AS
Date of Curation	27-03-2010
References	PMC368229

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PolysacDB ID	1233
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Brucella abortus S19 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Core oligosaccharide is composed of mannose, glucose, 2-amino-2,6-dideoxyglucose (quinovosamine), 2-amino-2-deoxyglucose (GlcN),3-deoxy-D-manno-2-octulosonic acid (KDO), and unidentified sugars. The core is linked to the lipid A, which in contrast to most LPS studied, contains both GlcN and 2,3- diamino-2,3-dideoxyglucose (GlcN3N) as backbone sugars and amide- and ester-linked long-chain saturated (C16:0 to C18:0) and hydroxylated (3-OH-C12:0, to 29-OH-C30:0) fatty acids
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Acetone-killed B. abortus S19 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Baro-2
Antibody Type Class	IgM
Assay System	Enzyme Immunoassay,immunoblotting
Cross Reactivity	The Baro-2 MAb showed reactivity against LPS from E. coli Re, S. minnesota Re, S. typhimurium Rd2, and S. typhimurium Rdl
Proposed Epitope	3-deoxy-D-manno-2-octulosonic acid (KDO)seems to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This Mab may help in the elucidation of epitopic distribution of Brucella abortus LPS
Curator ID	AA + AS
Date of Curation	27-03-2010
References	PMC368229

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PolysacDB ID	2021
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-killed P. aeruginosa cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab FK-2E7
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and flow cytometry
Cross Reactivity	Mab FK-2E7 bound to strains of Homma serotype E, strains of serotype M and to strains of serotype A and G
Proposed Epitope	This Mab reacted with the core region of the LPS
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	19-12-2010
References	1564440

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PolysacDB ID	2022
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-killed P. aeruginosa cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MH-4H7
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and flow cytometry
Cross Reactivity	Mab MH-4H7 bound to P. aeruginosa strains of serotype A, F, G, H, K and M, but did not bind to any strains of serotype B, C, E and I. This Mab also cross-reacted with E. coli 026 : B6 LPS
Proposed Epitope	This Mab reacted with the core region of the LPS. L-rhamnose in the LPS core of serotype G was an immunodominant sugar recognized by OM-1D6
IEDB Epitope	N/A
Proposed Utility	This Mab showed a high level of protective activity against P.aeruginosa in an experimental infection model using normal mice
Curator ID	AA + AS
Date of Curation	19-12-2010
References	1564440

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PolysacDB ID	2023
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-killed P. aeruginosa cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab OM-lD6
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and flow cytometry
Cross Reactivity	This Mab bound to P. aeruginosa strains. OM-1D6 reacted with all strains of serotype G so far tested, and a few strains of serotype M
Proposed Epitope	This Mab reacted with the core region of the LPS. L-rhamnose in the LPS core of serotype G was an immunodominant sugar recognized by OM-1D6
IEDB Epitope	N/A
Proposed Utility	This Mab showed a high level of protective activity against P.aeruginosa in an experimental infection model using normal mice
Curator ID	AA + AS
Date of Curation	20-12-2010
References	1564440

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PolysacDB ID	2024
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-killed P. aeruginosa cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab NM-3G8
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting and flow cytometry
Cross Reactivity	This Mab bound to P. aeruginosa strains. NM-3G8 bound to only a few strains of serotype B and M
Proposed Epitope	This Mab reacted with the core region of the LPS
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	20-12-2010
References	1564440

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PolysacDB ID	2025
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Heat killed Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 360.7
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross Reactivity	The Mab cross-reacted with some of the other Pseudomonas spp such as P . Acidovorans, P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . stutzeri., but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be used for an immunological assay for detection of P. aeruginosa in respiratory secretions of cystic fibrosis patients
Curator ID	AA + AS
Date of Curation	20-12-2010
References	1588587

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PolysacDB ID	2026
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Heat killed Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 304.1.4
Antibody Type Class	IgG2b
Assay System	ELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross Reactivity	The Mab cross-reacted with some of the other Pseudomonas spp such as P . Acidovorans, P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . stutzeri., but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab demonstrated opsonophagocytic killing of 06 subtype strains in the presence of human peripheral blood leukocytes and fresh human serum. This Mab also showed in vivo protection
Curator ID	AA + AS
Date of Curation	21-12-2010
References	1588587

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PolysacDB ID	2027
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Heat killed Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 73.5
Antibody Type Class	IgG1
Assay System	ELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross Reactivity	The Mab cross-reacted with some of the other Pseudomonas spp such as P . Acidovorans, P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . Stutzeri and P. testosteroni but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab demonstrated opsonophagocytic killing of 06 subtype strains in the presence of human peripheral blood leukocytes and fresh human serum. This Mab also showed in vivo protection
Curator ID	AA + AS
Date of Curation	21-12-2010
References	1588587

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PolysacDB ID	2028
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Heat killed Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 61.32
Antibody Type Class	IgG3
Assay System	ELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross Reactivity	The Mab cross-reacted with some of the other Pseudomonas spp such as P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . stutzeri., but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab demonstrated opsonophagocytic killing of 06 subtype strains in the presence of human peripheral blood leukocytes and fresh human serum. This Mab also showed in vivo protection
Curator ID	AA + AS
Date of Curation	22-12-2010
References	1588587

Record - 11 of 43 [TOP]

PolysacDB ID	2029
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Heat killed Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 334.4
Antibody Type Class	IgG3
Assay System	ELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross Reactivity	The Mab cross-reacted with some of the other Pseudomonas spp such as P . Acidovorans, P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . stutzeri., but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab demonstrated opsonophagocytic killing of 06 subtype strains in the presence of human peripheral blood leukocytes and fresh human serum. This Mab also showed in vivo protection
Curator ID	AA + AS
Date of Curation	22-12-2010
References	1588587

Record - 12 of 43 [TOP]

PolysacDB ID	2035
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin killed whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab KN-2B11
Antibody Type Class	IgM
Assay System	Competitive enzyme immunoassay, ELISA, immunoblotting
Cross Reactivity	"This MAb specifically bound to clinical isolates belonging to Homma serotypes A, F, G and K at a frequency of 70 to 86% and to serotypes H and M isolates at about 50%. They did not bind to any isolates of serotype B, E, and I tested."
Proposed Epitope	α-L-rhamnose residues in the outer core of LPS might be the part of the epitope.
IEDB Epitope	N/A
Proposed Utility	This Mab agglutinated P. aeruginosa cells
Curator ID	AA + AS
Date of Curation	23-12-2010
References	PMC313341

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PolysacDB ID	2036
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin killed whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab RM5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	"This Mab reacted specifically with P. aeruginosa Fisher immunotypes 2, 4, and 5"
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab protected neutropenic mice prophylactically against challenge with P. aeruginosa immunotypes 2, 4, and 5,
Curator ID	AA + AS
Date of Curation	24-12-2010
References	PMC259495

Record - 14 of 43 [TOP]

PolysacDB ID	2037
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin killed whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab FDD7
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	"This Mab reacted specifically with P. aeruginosa Fisher immunotypes 2, 4, and 5"
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab protected neutropenic mice prophylactically against challenge with P. aeruginosa immunotypes 2, 4, and 5,
Curator ID	AA + AS
Date of Curation	24-12-2010
References	PMC259495

Record - 15 of 43 [TOP]

PolysacDB ID	2038
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin killed whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 11F9
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	"This Mab reacted specifically with P. aeruginosa Fisher immunotypes 2, 4, and 5"
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab protected neutropenic mice prophylactically against challenge with P. aeruginosa immunotypes 2, 4, and 5,
Curator ID	AA + AS
Date of Curation	25-12-2010
References	PMC259495

Record - 16 of 43 [TOP]

PolysacDB ID	2039
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin killed whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 9H10
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	"This Mab recognized P. aeruginosa immunotypes 3, 6, and 7"
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab protected against P. aeruginosa immunotypes 3 and 6 but did not protect against immunotype 7
Curator ID	AA + AS
Date of Curation	25-12-2010
References	PMC259495

Record - 17 of 43 [TOP]

PolysacDB ID	2216
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella choleraesuis SN57 [Ra] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain
BCSDB Structure	N/A
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MASC1-MM3
Antibody Type Class	IgM
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab cross-reacted with with six different Salmonella core chemotypes S, Ra, Rb1, Rb2, Rb3, Rc and Rd1, the R2 core of Escherichia coli and a variety of smooth LPS
Proposed Epitope	The terminal disaccharide L-α-D-Hep1-->7-L-α-D-Hep1-O-me seemed to be the immunodominant epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	08-03-2011
References	1383323

Record - 18 of 43 [TOP]

PolysacDB ID	2217
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella minnesota Re chemotype 595 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The core oligosaccharide part consists of the following residues : α-Kdop-(2-8)-α-Kdop-(2-->4)-Kdo
BCSDB Structure	147530
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MASM1
Antibody Type Class	IgG2b
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab cross-reacted with Rd2 chemotype
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	08-03-2011
References	1383323

Record - 19 of 43 [TOP]

PolysacDB ID	2218
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella minnesota Re chemotype 595 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The core oligosaccharide part consists of the following residues : α-Kdop-(2-8)-α-Kdop-(2-->4)-Kdo
BCSDB Structure	147530
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MASM2
Antibody Type Class	IgG2b
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab was specific to Re chemotype
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	08-03-2011
References	1383323

Record - 20 of 43 [TOP]

PolysacDB ID	2219
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium SL 1181 [Rd2] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain
BCSDB Structure	N/A
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MAST20
Antibody Type Class	IgG2a
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab cross-reacted with Rd1 chemotype
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	09-03-2011
References	1383323

Record - 21 of 43 [TOP]

PolysacDB ID	2220
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium SL 1032 [Rd1] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The core oligosaccharide part consists of the following residues : L-gro-α-D-manHepp-(1-->7)-L-gro-α-D-manHepp-(1-->3)-L-gro-α-D-manHepp-(1-->5)-α-Kdop [branched to α-Kdop-(2-->4)] -(2--/lipid A/
BCSDB Structure	1784
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Heat killed bacteria
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MAST27
Antibody Type Class	IgG2b
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab was specific to Rd1 chemotype
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	10-03-2011
References	1383323

Record - 22 of 43 [TOP]

PolysacDB ID	2221
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium SL 805 [Rc] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain
BCSDB Structure	N/A
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MAST30
Antibody Type Class	IgG2b
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab cross-reacted with Rb3 chemotype
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	10-03-2011
References	1383323

Record - 23 of 43 [TOP]

PolysacDB ID	2222
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium TV 148 [Rb3] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain
BCSDB Structure	N/A
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Heat killed bacteria
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MAST41
Antibody Type Class	IgM
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab cross-reacted with Rb2 chemotype
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	10-03-2011
References	1383323

Record - 24 of 43 [TOP]

PolysacDB ID	2223
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium TV 161 [Rb2] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain
BCSDB Structure	N/A
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Heat killed bacteria
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MAST52
Antibody Type Class	IgG3
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab was specific to Rb2 chemotype
Proposed Epitope	N/A
IEDB Epitope	76694
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	10-03-2011
References	1383323

Record - 25 of 43 [TOP]

PolysacDB ID	2224
Carbohydrate Name	Core oligosaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella typhimurium SL 733 [Rb1] (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain
BCSDB Structure	N/A
Proposed Function	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature	Heat killed bacteria
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab MAST60
Antibody Type Class	IgG3
Assay System	Enzyme immunoassay, immunoblotting
Cross Reactivity	This Mab cross-reacted with Rb2 chemotype
Proposed Epitope	N/A
IEDB Epitope	76694
Proposed Utility	This Mab would help elucidate the LPS core domain structure
Curator ID	AA + AS
Date of Curation	11-03-2011
References	1383323

Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036