PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1067
Carbohydrate NameEnterobacterial common antigen   (Drugpedia)
Carbohydrate ClassGlycoprotein
Microbe NameEscherichia coli C600   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a glycophospholipid built up by an amino sugar heteropolymer linked to an L-glycerophosphatidyl residue.
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureWhole formalinized E. coli C600 cells
Carrier NameNil
Conjugate MethodNil
AntibodiesMab 865
Antibody Type ClassIgM
Assay SystemELISA and immunoblotting
Cross ReactivityThis antibody cross-reacted with purified ECA from S.montevideo and to E. coli Rl and R4 LPS, cross-reacted well with the K5 capsular polysaccharide
Proposed EpitopeEpitope was proposed to contain 4-linked α-N-acetylglucosamine
IEDB EpitopeN/A
Proposed UtilityThis Mab may be used for the detection of ECA and K5 core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator IDAA + AS
Date of Curation20-01-2010
ReferencesPMC261975

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PolysacDB ID1068
Carbohydrate NameEnterobacterial common antigen   (Drugpedia)
Carbohydrate ClassGlycoprotein
Microbe NameEscherichia coli C600   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a glycophospholipid built up by an amino sugar heteropolymer linked to an L-glycerophosphatidyl residue.
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureWhole formalinized E. coli C600 cells
Carrier NameNil
Conjugate MethodNil
AntibodiesMab 898
Antibody Type ClassIgG2a
Assay SystemELISA and immunoblotting
Cross ReactivityThis antibody did not cross-react with K5 capsular polysaccharide
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab may be used for the detection of ECA core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator IDAA + AS
Date of Curation20-01-2010
ReferencesPMC261975

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