PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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PolysacDB ID	1251
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Burkholderia pseudomallei NCTC 4845 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	Polysaccharide capsule of B. pseudomallei plays a role in environmental protection, immune system evasion and attachment to epithelial cells
Antigenic Nature	Heat inactivated B. pseudomallei NCTC 4845
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 4VIIIAII
Antibody Type Class	IgG2a
Assay System	ELISA and immunoblotting
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb demonstrated a significant protective effect against a challenge dose of B. Pseudomallei in mice
Curator ID	AA + AS
Date of Curation	09-04-2010
References	12466403

Record - 2 of 12 [TOP]

PolysacDB ID	1252
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Burkholderia pseudomallei NCTC 4845 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	Polysaccharide capsule of B. pseudomallei plays a role in environmental protection, immune system evasion and attachment to epithelial cells
Antigenic Nature	Heat inactivated B. pseudomallei NCTC 4846
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3VIE5
Antibody Type Class	IgG2b
Assay System	ELISA and immunoblotting
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb demonstrated a significant protective effect against a challenge dose of B. Pseudomallei in mice
Curator ID	AA + AS
Date of Curation	09-04-2010
References	12466403

Record - 3 of 12 [TOP]

PolysacDB ID	1253
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Burkholderia pseudomallei NCTC 4845 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	Polysaccharide capsule of B. pseudomallei plays a role in environmental protection, immune system evasion and attachment to epithelial cells
Antigenic Nature	Heat inactivated B. pseudomallei NCTC 4847
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 4VA5
Antibody Type Class	IgG1
Assay System	ELISA and immunoblotting
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb demonstrated a significant protective effect against a challenge dose of B. Pseudomallei in mice
Curator ID	AA + AS
Date of Curation	09-04-2010
References	12466403

Record - 4 of 12 [TOP]

PolysacDB ID	2051
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Burkholderia pseudomallei (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	It is a virulence determinant
Antigenic Nature	Heat inactivated whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3015
Antibody Type Class	IgG1
Assay System	ELISA, immunoblotting, immunoelectron microscopy
Cross Reactivity	This Mab cross-reacts only with Burkholderia mallei and other Burkholderia pseudomallei species
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	The isolated MAb will help to elucidate the role of antibodies against the exopolysaccharide in the defense of B. pseudomallei in experimental models
Curator ID	AA + AS
Date of Curation	30-12-2010
References	PMC173556

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PolysacDB ID	2339
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco1
Antibody Type Class	IgM
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	06-04-2011
References	7686001

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PolysacDB ID	2340
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco2
Antibody Type Class	IgG2b
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab cross-reacted with the closely related species S. benthica
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	06-04-2011
References	7686001

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PolysacDB ID	2341
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco3
Antibody Type Class	IgG2a
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab cross-reacted with the closely related species S. benthica
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

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PolysacDB ID	2342
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco4
Antibody Type Class	IgG2b
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Record - 9 of 12 [TOP]

PolysacDB ID	2343
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco5
Antibody Type Class	IgG2a
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Record - 10 of 12 [TOP]

PolysacDB ID	2344
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco6
Antibody Type Class	IgG1
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	07-04-2011
References	7686001

Record - 11 of 12 [TOP]

PolysacDB ID	2345
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco22
Antibody Type Class	IgG3
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	09-04-2011
References	7686001

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PolysacDB ID	2346
Carbohydrate Name	Exopolysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Shewanella colweliana (NCBI Taxonomy) (Drugpedia)
Basic Structure	The EPS is composed of mannose, glucose, galactose, and pyruvate in a 1:2:2:4 molar ratio. Molecular weight analysis by gel filtration demonstrates that the EPS is polydispersive and composed of a major high-molecularweight species and lesser amounts of smaller molecularweight species
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Aco23
Antibody Type Class	IgG1
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This Mab was specific to EPS of Shwenella colweliana
Proposed Epitope	This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to quantitate the synthesis and probe the function of S. colwelliana EPS
Curator ID	AA + AS
Date of Curation	09-04-2011
References	7686001

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