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| PolysacDB ID | 1037 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units |
| BCSDB Structure | N/A |
| Proposed Function | Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs |
| Antigenic Nature | Formalinized spores of A. fumigatus. |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab EB-A2 |
| Antibody Type Class | IgM |
| Assay System | Indirect and Competitive ELISA |
| Cross Reactivity | This antibody cross-reacted to P. digitatum and Penicillium marneffei speices. |
| Proposed Epitope | 3(1-->5)galactofuranose residues of galactomannan |
| IEDB Epitope | N/A |
| Proposed Utility | EB-A2 has been used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition. The sensitivity (95%), specificity (100%), positive predictive value (100%), and negative predictive value (99%)of the ELISA |
| Curator ID | AA + AS |
| Date of Curation | 10-01-2010 |
| References | PMC257149 |
| PolysacDB ID | 1043 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units |
| BCSDB Structure | N/A |
| Proposed Function | Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs |
| Antigenic Nature | Cold alkali extract of mycelium |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 40 |
| Antibody Type Class | IgM |
| Assay System | Indirect Enzyme Immunoassay, immunofluorescence, Preciptin assay |
| Cross Reactivity | This antibody was cross-reactive with C.albicans |
| Proposed Epitope | Mannopyranosyl side chain |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody can be used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition. |
| Curator ID | AA + AS |
| Date of Curation | 13-01-2010 |
| References | PMC258783 |
| PolysacDB ID | 1044 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units |
| BCSDB Structure | N/A |
| Proposed Function | Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs |
| Antigenic Nature | Cold alkali extract of mycelium |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab 1 |
| Antibody Type Class | IgM |
| Assay System | Indirect Enzyme Immunoassay, immunofluorescence, Preciptin assay |
| Cross Reactivity | This antibody was cross-reactive with C.albicans |
| Proposed Epitope | Oligogalactoside side chain |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody can be used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition. |
| Curator ID | AA + AS |
| Date of Curation | 13-01-2010 |
| References | PMC258783 |
| PolysacDB ID | 1129 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 701 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | Formalinized A. flavus EV 701 spores |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-1 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis |
| Curator ID | AA + AS |
| Date of Curation | 08-02-2010 |
| References | PMC257149 |
| PolysacDB ID | 1130 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 601 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | A mycelial extract of A. fumigatus EV 601 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-2 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | EB-A2 has been used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition |
| Curator ID | AA + AS |
| Date of Curation | 09-02-2010 |
| References | PMC257149 |
| PolysacDB ID | 1131 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 601 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | A mycelial extract of A. fumigatus EV 601 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-3 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis |
| Curator ID | AA + AS |
| Date of Curation | 09-02-2010 |
| References | PMC257149 |
| PolysacDB ID | 1132 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 601 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | Formalinized spores of A. fumigatus EV 601 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-4 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis |
| Curator ID | AA + AS |
| Date of Curation | 10-02-2010 |
| References | PMC257149 |
| PolysacDB ID | 1133 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 601 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | A mycelial extract of A. fumigatus EV 601 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-5 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis |
| Curator ID | AA + AS |
| Date of Curation | 10-02-2010 |
| References | PMC257149 |
| PolysacDB ID | 1134 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 701 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | A mycelial extract of A. flavus EV 701 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-6 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis |
| Curator ID | AA + AS |
| Date of Curation | 11-02-2010 |
| References | PMC257149 |
| PolysacDB ID | 1135 |
| Carbohydrate Name | Galactomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Aspergillus flavus EV 701 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Galactomannans are composed of an α-1,6-linked mannan backbone substituted with mannopyranose oligosaccharides and immunodominant terminal galactofuranose present either singly or linked to oligogalactosides |
| BCSDB Structure | N/A |
| Proposed Function | The wide distribution and similar chemical structure of the galactomannans have been responsible for serologic cross-reactions |
| Antigenic Nature | A mycelial extract of A. flavus EV 701 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab EBA-7 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Immunofluorescence and immunoblotting |
| Cross Reactivity | This Mab cross-reacted with the five Aspergillus species (A. fumigatus ATCC 1028, A. flavus ATCC 10124, A. niger ATCC 10549, A. versicolor ATCC 16845, and A. terreus CBS 106.25) but not with other medically important fungi, such as Sporothrix schenckii ATCC 14096 and Candida spp. (25 strains, including 10 ATCC strains, 2 CBS strains, and 13 clinical isolates, representing seven species, i.e., C. albicans, C. tropicalis, C. [Torulopsis] glabrata, C. pseudotropicalis, C. parapsilosis, C. guilliennondii, and C. krusei). They also failed to react with the 15 different bacterial species tested |
| Proposed Epitope | An epitope located on the β(1-->5) galactofuranose containing side chains of the galactomannan molecule |
| IEDB Epitope | N/A |
| Proposed Utility | This rat Mab can be used against A. fumigatus galactomannan, already applied to the serological and immunohistological diagnosis of invasive aspergillosis |
| Curator ID | AA + AS |
| Date of Curation | 12-02-2010 |
| References | PMC257149 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |