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| PolysacDB ID | 1001 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 116826 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates. |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA), tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Polysera |
| Antibody Type Class | IgM |
| Assay System | Double immunodiffusion, ELISA |
| Cross Reactivity | Polsera cross-reacted with capsular polysaccharides of C neoformans serotype A and D |
| Proposed Epitope | O-acetyl groups, glucuronyl residues |
| IEDB Epitope | 115576 |
| Proposed Utility | The conjugate vaccines prepared through hydroxyl activation of glucurunoxylomannan are sufficiently immunogenic and appear to be suitable for clinical evaluation |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | 1716613 |
| PolysacDB ID | 1002 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates. |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 2E9 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A, B and D |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | PMC173409 |
| PolysacDB ID | 1006 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear (1-->3)-linked mannan backbone singly substituted with nonreducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 3B6 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotypes A, B, C and D |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | Human glucurunoxylomannan monoclonal antibodies are essential to address questions regarding the role of glucurunoxylomannan antibodies in protection against cryptococcal infections |
| Curator ID | AA + AS |
| Date of Curation | 02-01-2010 |
| References | PMC173409 |
| PolysacDB ID | 1013 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM backbone consists of a linear α(1-->3)-linked mannan substituted at 2-O positions by single residues of either xylose or glucuronic acid |
| BCSDB Structure | 116826 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Pure Capsular polysaccharide |
| Carrier Name | N/A |
| Conjugate Method | N/A |
| Antibodies | Mab E1 |
| Antibody Type Class | IgG1 |
| Assay System | Agglutination with C. neoformans serotype A cells, Radial immunodiffusion, Indirect Immunofluorescence,, ELISA, Competitive binding assays |
| Cross Reactivity | This antibody was highly specific to serotype A, low levels of cross reactivity to serotypes B and D. Among the other yeasts tested, a cross-reaction was only detected with Trichosporon beigelii. |
| Proposed Epitope | N\A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could be useful for fundamental studies on the glucuronoxylomannan structure, as well as for clinical applications such as serotyping and possibly the serological diagnosis of cryptococcosis |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC260404 |
| PolysacDB ID | 1028 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
| BCSDB Structure | 130362 |
| Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugate Method | GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg] |
| Antibodies | Mab BD1 |
| Antibody Type Class | IgG1 |
| Assay System | Indirect immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and D. BD-1 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus |
| Proposed Epitope | Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC259921 |
| PolysacDB ID | 1029 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
| BCSDB Structure | 130362 |
| Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Bovine serum albumin |
| Conjugate Method | GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg] |
| Antibodies | Mab BA4 |
| Antibody Type Class | IgM |
| Assay System | Indirect immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and B. BA4 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC259921 |
| PolysacDB ID | 1031 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A |
| BCSDB Structure | 130362 |
| Proposed Function | The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 18B7 |
| Antibody Type Class | IgG1 |
| Assay System | Invivo protection assay |
| Cross Reactivity | This antibody cross-reacted with all 4 serotypes- A, B, C and D. |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody opsonized Serotypes A and D and was shown to activate the complement pathway. This antibody is in pre-clinical development for treatment of Cryptococcus neoformans infections |
| Curator ID | AA + AS |
| Date of Curation | 09-01-2010 |
| References | PMC105619 |
| PolysacDB ID | 1496 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues |
| BCSDB Structure | N/A |
| Proposed Function | C. neoformans has a large polysaccharide capsule that is a determinant of virulence. The capsular polysaccharide causes immunologic paralysis and inhibits phagocytosis by macrophages. Soluble C. neoformans capsular polysaccharide enhances human immunodeficiency virus [HIV] infection in cultured cells, suggesting a new pathogenic role in AIDS |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was conjugated to tetanus toxoid using CDAP precipitation |
| Antibodies | Polysera |
| Antibody Type Class | IgG [predominantly IgG1] |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype D strains |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | N/A |
| Curator ID | AA + AS |
| Date of Curation | 04-08-2010 |
| References | 1583327 |
| PolysacDB ID | 2606 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 2D10 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed Epitope | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2607 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 3E5 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed Epitope | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2609 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 17E12 |
| Antibody Type Class | IgG1 |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed Epitope | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2610 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 18G9 |
| Antibody Type Class | IgA |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed Epitope | This antibody did not bind O-deacetylated glucurunoxylomannan indicating that acetylated residues on glucurunoxylomannan surface are an essential part of the epitope |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 17-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2611 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Tetanus toxoid |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 2H1 |
| Antibody Type Class | IgG1 |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A,B,C and D |
| Proposed Epitope | This antibody did not bind O-deacetylated glucurunoxylomannan indicating that acetylated residues on glucurunoxylomannan surface are an essential part of the epitope |
| IEDB Epitope | 115576 |
| Proposed Utility | Could help in specific serotype identification |
| Curator ID | AA + AS |
| Date of Curation | 17-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2612 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 1326 |
| Antibody Type Class | IgG1 |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A and D |
| Proposed Epitope | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | This monoclonal antibody promoted opsonization of Cryptococcus cells |
| Curator ID | AA + AS |
| Date of Curation | 18-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2617 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 4H9 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 21-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2618 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 6C3.4 |
| Antibody Type Class | IgM |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 22-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2619 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 6A5 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 22-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2620 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 9A12 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 22-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2621 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab A2F12 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 23-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2622 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 6C3.3 |
| Antibody Type Class | IgG1 |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 23-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2623 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab IG5 |
| Antibody Type Class | IgG2a |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 24-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2624 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 7f8 |
| Antibody Type Class | IgG2a |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 24-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2625 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 12g6 |
| Antibody Type Class | IgG2a |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 24-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2626 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 12A1.4 |
| Antibody Type Class | IgG2b |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 24-07-2011 |
| References | 10456888 |
| PolysacDB ID | 2627 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 130362 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | GXM was derivatized by the following two methods. (i) In method 1, ADH [adipic acid dihydrazide] was introduced into GXM by the activation of carboxyl groups with EDAC. GXM (5 mg/ml of 0.2 M NaCl) was derivatized with 0.5 M ADH and 0.1 M EDAC at pH 4.85 for 3.5 h at room temperature, using a pH Stat. After extensive dialysis against 0.2 M NaCl, the reaction mixture was passed through a 2B-CL Sepharose column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The fractions containing GXM were pooled and concentrated to the original volume. (ii) In method 2, ADH was introduced into GXM by the activation of hydroxyl groups with CNBr. GXM (5 mg/ml of 0.2 M NaCl) was activated with an equal weight of CNBr at pH 10.5 for 6 min at 4°C, using a pH Stat. An equal volume of 0.5 M NaHCO3 (pH 8.5) containing 0.5 M ADH was added. The reaction mixture was tumbled at 3 to 8°C for 18 to 20 h, dialyzed against 0.2 M NaCl, and passed through a 2B-CL Sepharose column (1.5 by 30 cm). The fractions containing GXM were pooled and concentrated to the original volume. The reaction mixture, containing equal concentrations (3.0 to 7.5 mg/ml) of GXM-AH (derivatized by either method) and TT or rEPA in 0.2 M NaCl, was brought to pH 5.6 with 0.05 N HCl, and 0.05 to 0.1 M EDAC was added; the pH was maintained at 5.6 in a pH Stat for 1 to 3 h at 4°C. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a Sepharose 2B-CL column (1.5 by 30 cm) equilibrated in 0.2 M NaCl. The void volume fractions containing the GXM and the protein were pooled and stored in 0.01% thimerosal at 3 to 8°C |
| Antibodies | Mab 15E6 |
| Antibody Type Class | IgG2b |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used to decipher and differentiate the serum antibody response of autoimmune mice as compared to normal mice |
| Curator ID | AA + AS |
| Date of Curation | 24-07-2011 |
| References | 10456888 |
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Bioinformatics Centre, Institute of Microbial Technology, Sec - 39A, Chandigarh, India - 160036 |