PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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PolysacDB ID	1093
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (reference strain M62) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 6D11
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab showed a high degree of reactivity with serotypes 4 and 7 and also detected some isolates belonging to serotypes 1, 2, 3, 6, and 8, as well as some nontypeable isolates
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	28-01-2010
References	PMC170200

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PolysacDB ID	1094
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (reference strain M62) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 6B4
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab reacted with only 24.2 and 13.8% of homologous isolates, respectively, but detected 63% of the serotype 7 isolates. A minor reactivity was observed with other serotypes
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	28-01-2010
References	PMC170200

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PolysacDB ID	1095
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (reference strain M62) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5E4
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab reacted with only 24.2 and 13.8% of homologous isolates, respectively, but detected 63% of the total 12 serotype 7 isolates. A minor reactivity was observed with other serotypes
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	28-01-2010
References	PMC170200

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PolysacDB ID	1103
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5C5
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	30-01-2010
References	PMC170200

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PolysacDB ID	1104
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 1E10
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	30-01-2010
References	PMC170200

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PolysacDB ID	1108
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5H7
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	31-01-2010
References	PMC170200

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PolysacDB ID	1143
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab QUBf5
Antibody Type Class	IgG2b
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross Reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE3
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	15-02-2010
References	1719202

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PolysacDB ID	1144
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab QUBf6
Antibody Type Class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross Reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE4
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	15-02-2010
References	1719202

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PolysacDB ID	1145
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab QUBf7
Antibody Type Class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross Reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE5
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-02-2010
References	1719202

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PolysacDB ID	1146
Carbohydrate Name	High molecular weight polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	-3)α-D-FucpNAc4N(1-->4)α-D-GalpNAc [branched to β-D-Galf(1-->3)] (1-->3)β-D-Galp [branched via 6th and 4th positions to RPyr(2-(1-.C73
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab QUBf8
Antibody Type Class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross Reactivity	This Mab did not cross-react with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. But it reacted with B. fragilis strain BE6
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	16-02-2010
References	1719202

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