| PolysacDB ID | 1091 |
| Carbohydrate Name | Inner core Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Escherichia coli F653 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It consists of the uncommon sugars L-glycero-D mannoheptose (Hep) and 3-deoxy-D-manno-octulosonic acid (Kdo) along with Lipid A
|
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Heat killed bacteria |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab WN1 222-5 |
| Antibody Type Class | IgG2a |
| Assay System | ELISA, immunoblotting, passive hemolysis assay |
| Cross Reactivity | WN1 222-5 binds to all E. coli clinical isolates tested so far (79 blood isolates, 80 urinary isolates, and 21 fecal isolates), Salmonella LPS, Shigella LPS and to some Citrobacter, Enterobacter, and KiebsieUla isolates |
| Proposed Epitope | The epitope consists of the inner core |
| IEDB Epitope | 76794 |
| Proposed Utility | In vitro, this Mab inhibits the Limulus amoebocyte lysate assay activity of S-form and R-form LPS in a dose-dependent manner and it neutralizes the LPS-induced release of clinically relevant monokines (interleukin 6 and tumor necrosis factor). In vivo, WN1 222-5 blocks endotoxin-induced pyrogenicity in rabbits and lethality in galactosamine-sensitized mice |
| Curator ID | AA + AS |
| Date of Curation | 27-01-2010
|
| References | PMC281087
|